生态学杂志2011，Vol.30Issue(6)：1234-1239,6.
镉胁迫对拟南芥幼苗基因组DNA多态性的影响
Effects of cadmium stress on DNA polymorphism of genome in Arabidopsis thaliana seedlings
摘要
Abstract
By using random amplified polymorphic DNA ( RAPD ) technique , the DNA polymorphism of genome in Arabidopsis thaliana seedlings leaves under Cd stress was studied, and, in combining with leaf morphological and physiological indices, the sensitive biomarkers indicative of Cd stress were chosen. After treated with 0. 125 -3. 0 mg Cd · L-1 for 21 days, the soluble protein content in A. thaliana seedlings leaves had an inverted U-shape increase with increasing Cd concentration, but no significant changes were observed in leaf fresh weight and chlorophyll content. Of the 12 decamer oligonucleotide primers tested, only 5 gave specific and stable results. In the control, 44 bands of the RAPD fingerprints from the leaves were easily distinguished. The changes in the RAPD fingerprints under Cd stress included the variation of band intensity, loss of normal bands, and appearance of new bands, in comparison with the control. In addition, the changes were dose-dependent. The above results showed that the genomic template stability was significantly affected by Cd stress, and the DNA polymorphism was sensitive to Cd stress. Thereby, the DNA polymorphism detected by RAPD technique could be used as a potential biomarker assay for the detection of Cd pollution and its related biological effects.关键词
拟南芥/Cd/DNA多态性/RAPDKey words
Arabidopsis thaliana / Cd / DNA polymorphism / RAPD.分类
农业科技引用本文复制引用
詹振楠,刘宛,孙梨宗,王善仙,李培军,吴海燕..镉胁迫对拟南芥幼苗基因组DNA多态性的影响[J].生态学杂志,2011,30(6):1234-1239,6.基金项目
国家自然科学基金项目(20977095、40930739和21077113)和中国科学院知识创新工程重要方向项目(kzcx2-yw-446)资助. （20977095、40930739和21077113）
