中国农业科学2011，Vol.44Issue(8)：1553-1561,9.
桑属IIS、trnL-F、rps16序列与进化分析
Morus ITS, trnL-F, rps16 Sequence and Phylogenetic Analysis of Mulberry Resources
摘要
Abstract
[Objective] In this research, the sequences ofMorus-ITS, trnL-F, and rps16 were analyzed to discuss the value of phylogeny. [Method] A total of 67 mulberry genotypes were analyzed (DNA extraction, PCR amplification, sequencing, software splicing, alignment, removing non-sequence bases, downloading Morus ITS, trnL-F, rps16 sequences from GenBank and analyzing percentidentity, calculating length and G+C (A+T) content, mutant sites and informative sites), then these three sequences were combined. A cladogram, generated using MP, was built by using B. papyrifera and C. tricuspidata as outgroup. [Result] The length of basic sequence of mulberry ITS (including 5.8S) is 576 bp, variation range of 576-590 bp, G+C 59.55％-62.25％, 40 informative sites. Mulberry trnL-F intergenic sequence (including the tRNA-Leu intron), the length of basic sequence is 923 bp, variation range of 920-924 bp, A +T 65.87％-66.31％, 23 informative sites. The length of basic sequence ofrps16 intron is 929 bp, variation range of 923-929 bp, A+T 67.28％-67.49％, 17 informative sites. The most possible evolutionary models were (GTR+G), (GTR) and (GTR+G), respectively. And the optimal evolutionary model of the 3-segment-combined analysis was (GTR+G). In the cladogram,Morus nigra is separated first, the rest is divided into two branches. Branch Ⅰ of this cladogram consists of 8 species, including M. wittorum, M. mizuho, M. mongolica, M. mongolica var. diabolica, M. Australis, M. Bombycis, M. Alba and M. atropurprea. Other three members ( M. cathayana, M. Macroura, M. notabilis) falls under branch Ⅱ. [Conclusion] trnL-F and rps16 sequence information sites of Morus is limited, but combined with the ITS sequences, it can improve the branch map information sites, and make branch map more reliable. Based on ITS, trnL-F, rps16 sequence MP, M. nigra (Xinjiang) is divided into a single branch, as the most primitive type, while cultivated species such as Ukraine is the most diverged.关键词
桑属/ITS、trnL-F、rps16序列/MP分析法/进化关系Key words
Morus/ ITS/ trnL-F/ rps16 sequence/ MP analysis/ phylogeny relationship引用本文复制引用
陈仁芳,张泽,唐洲,余茂德,徐立,王茜玲..桑属IIS、trnL-F、rps16序列与进化分析[J].中国农业科学,2011,44(8):1553-1561,9.基金项目
重庆市重大攻关项目(cq-2009001)、西南大学博士启动基金(swu110059) （cq-2009001）
