渔业科学进展2011，Vol.32Issue(2)：83-88,6.
尿素对中华绒螯蟹幼蟹非特异性免疫功能的影响
Impact of urea on non-specific immune response of juvenile Eriocheir sinensis
摘要
Abstract
Urea is an important factor for paddy production in paddy-crab ecosystem.However, application of urea may impose negative effects on the growth of crabs. In our research,the effects of different concentrations of urea on the non-specific immune system of juvenile Eriocheir sinensis were studied in order to provide necessary theoretical support for setting up regulations for the paddy-crab ecosystem. The juvenile E. sinensis were reared in water containing 0. 24,0. 48,0. 72,0. 96 ,and 1.2 g/L urea respectively for 7 days without feeding. The activities of three enzymes including superoxide dismutase (SOD),catalase (CAT),and glutathione-S transferase (GST), as well as the content of malondialdehyde (MDA) in crab serum were determined. The results showed that the activities of SOD,CAT,and GST decreased gradually in crab serum for all urea concentrations during the experimental period,while the content of MDA increased instead. Also the activities of the above three enzymes showed a decreasing tendency as the urea concentration increased. However, the SOD activity in 0.24g/L and the GST activity in 0.96g/L urea treatments were significantly higher than those in the other ureatreated groups,while the content of MDA in these two treatments decreased. The results suggest that the added urea reduces the E. sinensis's capability of eliminating lipid peroxides, hydrogen peroxide and free radicals. As a result, the non-specific defense system was damaged with the application of urea.关键词
中华绒螯蟹/尿素/血淋巴/非特异性免疫功能Key words
Chinese mitten crab/Urea/Haemolymph/Non-specific immune response分类
农业科技引用本文复制引用
袁滢滢,吴朝霞,孙文涛,黄姗,范翠翠..尿素对中华绒螯蟹幼蟹非特异性免疫功能的影响[J].渔业科学进展,2011,32(2):83-88,6.基金项目
辽宁省科技厅农业攻关计划(2007203001)资助 （2007203001）
