基础医学与临床2011，Vol.31Issue(6)：642-646,5.
MicroRNA-144调控红系分化
MicroRNA-144 regulates erythroid differentiation of K562 cells
摘要
Abstract
Objective We aimed to study the influence of miR-144 on human erythroid differentiation via targeting the retinoblastoma protein (RB). Methods K562 cells were treated with hemoin to differentiate into mature red cells. Real-time PCR analysis was used to detect the changes of miR-144 expression during erythroid differentiation.K562 cells were transfected with oligonucleotides (mimic-l44) and the influence of erythropoiesis was measured. The target gene of miR-144 was identified by using bioinformatics analysis combined with Dual-luciferase reporter and Western blot analysis. Results miR-144 was significanfiy increased during hemoin-induced K562 erythroid differentiation (P < 0.05). Over-expression of miR-144 in K562 cells promoted the stimulation of γ-globin and CD235a. Moreover, RB was identified as a direct target of miR-144. Expression of RB increased slightly and then reduced obviously in erythroid differentiation. Grey mean of RB/GAPDH minimum point: 12 h 0.092 ±0.007(P <0.05 compared with 0 h). Conclusion miR-144 plays a positive role in erythroid differentiation of K562 cells via targeting RB.关键词
microRNA-144/红系分化/视网膜母细胞瘤蛋白Key words
microRNA-144/ erythroid differentiation/ the retinoblastoma protein分类
生物科学引用本文复制引用
汪晓艳,蒋崇亮,朱勇,王芳,余佳,冯涛..MicroRNA-144调控红系分化[J].基础医学与临床,2011,31(6):642-646,5.基金项目
国家自然科学基金(81071770) （81071770）
