基础医学与临床2011，Vol.31Issue(6)：647-651,5.
NR2F2促进小鼠MC3T3-E1前成骨细胞增殖
NR2F2 accelerates mouse MC3T3-E1 pre-osteoblast proliferation
摘要
Abstract
Objective To study the role of NR2F2 in murine pre-osteoblast proliferation.Methods Quantitative real-time PCR was used to measure the mRNA level, MTS assay was used to assess the cell proliferation rate, flow cytometry was used to figure out the cell cycle distribution, and ELISA-Brdu was used to check the rate of DNA synthesis.Results When murine pre-osteoblast MC3T3-E1 cell proliferation was accelerated, the expression level of NR2F2 remarkably increased to 4.57 ±0.30 ( P ＜0.0l ) fold compared with that of the control.Over-expression of NR2F2 accelerated MC3T3-E1 cell proliferation (P ＜0.01 ), and the proportion of cells in S phase markedly increased to 2 fold compared with that of the control, and the proportion of cells in G2/M phase also went up ( P ＜ 0.05 ).More Brdu was incorporated into NR2F2 over-expressing cells, indicating the increase of DNA replication rate (P ＜0.01 ).Conclusion NR2F2 accelerates murine pre-osteoblast MC3T3-E1 proliferation through increasing the proportion of cells in S phase.关键词
NR2F2/MC3T3-E1细胞/细胞增殖Key words
NR2F2/ MC3T3-E1 cell/ cell proliferation分类
医药卫生引用本文复制引用
王崧,熊霞辉,朱宁,陈梅红..NR2F2促进小鼠MC3T3-E1前成骨细胞增殖[J].基础医学与临床,2011,31(6):647-651,5.基金项目
国家自然科学基金(90608023) （90608023）
国家(重点)实验室专项经费(2060204) （重点）
