基础医学与临床2011，Vol.31Issue(6)：667-671,5.
埃博拉病毒包膜蛋白GP基因DNA疫苗诱导小鼠产生高滴度中和抗体
DNA vaccine encoding Ebola virus envelope glycoprotein inducing high-titer neutralization antibody in mice
摘要
Abstract
Objective To characterize of neutralization antibody in mice induced by DNA vaccine encoding Zaire Ebolavirus glycoprotein (GP) and to develop DNA vaccine against Ebola virus. Methods Zaire Ebolavirus GP encoding plasmid Peak13CD5LGP was constructed. 100 μg dosage of Peak13CD5LGP was used to immunize the mice, the titer of neutralization antibody against Zaire Ebolavirus glycoprotein was assayed by EL1SA using Zaire Ebolavirus glycoprotein subunit GP1-Fc fusion protien as capture antigen. Results After boost immunization with 100 μg dosage Peak13CD5LGP plasmid, the titer of neutralization antibody of anti-GP was 1:2 000, both efficiency and specificity of neutralization antibody was verified by western blot. Conclusion 100 μg dosage of DNA vaccine encoding Zaire Ebolavirus envelope glycoprotein is able to induce persistent and high level of neutralizing anibody, and may be a potential vaccine against the deadly Zaire Ebolavirus.关键词
埃博拉病毒/包膜蛋白/DNA疫苗Key words
Zaire Ebolavirus/ envelope glycoprotein/ DNA vaccine分类
医药卫生引用本文复制引用
张桂根,郎建社,王洪亮,刘康泰,蒋澄宇..埃博拉病毒包膜蛋白GP基因DNA疫苗诱导小鼠产生高滴度中和抗体[J].基础医学与临床,2011,31(6):667-671,5.基金项目
国家自然科学基金(30625013) （30625013）
