基础医学与临床2011，Vol.31Issue(6)：683-687,5.
P38MAPK信号通路的激活促进SH-SY5Y细胞内Aβ生成
The activation of P38 MAPK signaling pathway increases intracellular production in SH-SY5Y
摘要
Abstract
Objective To investigate the effect of the activation of P38 MAPK signaling pathway on the intracellular β-amyloid(Aβ) production in human neuroblastoma SH-SY5Y. Methods SH-SY5Y cells were used to test intracellular Aβ levels by ELISA after activation of P38 MAPK signaling pathway by anisomycin for 1 and 72 h. The mRNA and protein levels of APP, PS1 and BACE1 were examined by RT-PCR and Western blot respectively. Results The expression of Phospho-P38 MAPK was increased after anisomycin treatment for 1 and 72 h; the production of Aβ1-40 was about ( 46. 88 ± 3.60) pg/mL[ Control group was (39.09 ± 1.60) pg/mL, P ＜ 0. 05 ] after anisomycin treatment for 72 h; APP and PS1 mRNA was increased after anisomycin treatment for 1 h, while APP,PS1 and BACE1 mRNA was significantly increased after anisomycin treatment for 72 h;the protein levels of APP,PS1 and BACE1 were increased as 1.45-fold, 1.38-fold and 1.60-fold respectively as control after anisomycin treatment for 72 h while nothing alter for 1 h. Conclusion Activation of P38 MAPK signaling pathway may increase Aβ production and play a role in the pathogensis of Alzheimer's disease (AD).关键词
阿尔茨海默病/P38MAPK信号通路/β-淀粉样肽Key words
Alzheimer's disease/ P38 MAPK/ β-Amyloid(Aβ).分类
医药卫生引用本文复制引用
郭学文,李良..P38MAPK信号通路的激活促进SH-SY5Y细胞内Aβ生成[J].基础医学与临床,2011,31(6):683-687,5.基金项目
北京市自然科学基金(7102012),北京市教育委员会科技计划(KM201110025006) （7102012）
