中国农业科学2011，Vol.44Issue(8)：1710-1719,10.
茶皂素和丝兰皂苷对山羊人工瘤胃发酵和瘤胃微生物的影响
Effect of Yucca schidigera Saponin and Tea Saponin Mixture on the Rumen Fermentation and Its Fibrolytic Bacterial Activity in the Rusitec Substrates with Different Concentrate to Forage Ratio
摘要
Abstract
[Objective]An experiment was designed to explore the effect of Yucca schidigera saponin and tea saponin mixture and different concentrate/forage ratios In vitro on rumen fermentation and rumen fiberolytic bacterial activity.[Method]A 2×4 two factorial design of experiment was adopted: one factor was adding or without saponins (0.3％ tea saponin and 0.03％ Yucca schidigera extract), the other was four different concentrate/forage ratios (20∶ 80, 40∶ 60, 50∶ 50, and 60∶ 40).[Result]The results showed that the mixed saponins reduced pH and the group whose concentrate/forage ratio was 20∶ 80 had significant effect (P＜0.05).Concentrations of acetate, propionate, butyrate and total VFAs were increased by addition of the mixed saponins, however,from the molar ratio of VFAs it was found that the proportion of propionate and butyrate were decreased while the proportion of propionate was increased, so the acetate/propionate ratio descended.The mixed saponins increased ruminal bacterial protein yield whereas the yield of ruminal protozoal protein was decreased, and the ratio of bacterial protein/protozoal protein was significantly increased (P＜0.05) compared with the control group.The relative amount of Fibrobacter succinogenes was extremely higher by addition of the mixed saponins whose concentrate/forage ratio was not 20∶ 80.And the mixed saponins increased the relative amount of Ruminococcus flavefaciens and Ruminococcus albus except that the concentrate/forage ratio was 40∶ 60.[Conclusion]Results indicate that this mixed saponins have effects on microbial fermentation and fibroliytic bacteria activities, and that the effects depend on the ratio of concentrate to rughage in the substrate.关键词
茶皂素/丝兰皂苷/瘤胃发酵/瘤胃微生物Key words
Yucca schidigera saponin/ tea saponin/ rumen fermentation/ fibrolytic bacterial activity引用本文复制引用
王洪荣,陈旭伟,王梦芝..茶皂素和丝兰皂苷对山羊人工瘤胃发酵和瘤胃微生物的影响[J].中国农业科学,2011,44(8):1710-1719,10.基金项目
国家自然科学基金项目(30771567) （30771567）
