中国农业科学2011，Vol.44Issue(8)：1736-1744,9.
紫心甘薯二氢黄酮醇4-还原酶基因表达及酶活性与花色苷积累的相关性
Correlations Among the Enzyme Activity and Gene Expression of Dihydroflavonol 4-Reductase and Anthocyanin Accumulation in Purple-Fleshed Sweet Potato
摘要
Abstract
[Objective] Two purple-fleshed sweet potato cultivar and strain, ‘A5’ and ‘Yamakawamurasaki’, and one white-fleshed sweet potato cultivar ‘Yubeibai’ were used to study the role of dihydroflavonol 4-reductase (DFR) in anthocyanin accumulation of purple- fleshed sweet potato. [ Method ] Anthocyanin content, DFR activities and expression of IbDFR gene were determined respectively, while the correlation between the content of anthocyanin and DFR activities, and that between anthocyanin content and IbDFR gene expression were analyzed. [ Result ] The results showed that the activities of DFR were dramatically and positively correlated with the content of anthocyanin no matter in different organs of the same cultivar (strain) or in same organ of the same cultivar (strain) at different growth stages. Moreover, the variation trends of the relative expression of IbDFR gene also coincided with that of anthocyanin content in storage roots of different cultivars (strain) and of different developing states of ‘Yamakawamurasaki’. [Conclusion] These results indicate that DFR is a key enzyme in anthocyanin synthesis of purple- fleshed sweet potato, and the biosynthesis of anthocyanin is in situ synthesis in purple-fleshed sweet potato.关键词
紫心甘薯/花色苷/二氢黄酮醇4-还原酶/相关性分析Key words
purple-fleshed sweet potato/ anthocyanin/ dihydroflavonol 4-reductase/ correlation analysis引用本文复制引用
郭晋雅,李云萍,傅玉凡,黄成涛,周伟,高峰..紫心甘薯二氢黄酮醇4-还原酶基因表达及酶活性与花色苷积累的相关性[J].中国农业科学,2011,44(8):1736-1744,9.基金项目
国家自然科学基金资助项目(31040060)、广东省自然科学基金资助项目(10151063101000038) （31040060）
