中国组织工程研究与临床康复2011，Vol.15Issue(23)：4282-4286,5.DOI:10.3969/j.issn.1673-8225.2011.23.023
远志总皂苷干预β-淀粉样蛋白致伤神经干细胞Caspase-3及Par-4的表达
Effect of tenuigenin on the Caspase-3 and Par-4 expression of neural stem cells induced by beta-amyloid protein
摘要
Abstract
BACKGROUND: The effect of tenuigenin which has a good nerve protection to neural stem cells has not been reported. OBJECTIVE: To investigate the protective effect and mechanism of tenuigenin for neural stem cells in the hippocampus impaired by β-amyloid protein.METHODS: The third passage neural stem cells generated from the hippocampi of Kunming mice which injured by β-amyloid protein in vitro were incubated with different concentrations of tenuigenin.RESULTS AND CONCLUSION: Immunocytochemical technique was used to detect Caspase3-positive neural stem cells. The study revealed that the expression of Par-4 and caspase-3 positive neural stem cells in the tenuigenin group were significantly lower than that in the control group with statistical significance (P ＜ 0.05). Tenuigenin can reduce the expression of Par-4 and Caspase-3 in neural stem cells impaired by β-amyloid protein.关键词
远志总皂苷/神经干细胞/Caspase-3/Par-4/β-淀粉样蛋白分类
基础医学引用本文复制引用
张晓梅,孙光涛,黄作义,吴成吉,戚询中,朱晓峰..远志总皂苷干预β-淀粉样蛋白致伤神经干细胞Caspase-3及Par-4的表达[J].中国组织工程研究与临床康复,2011,15(23):4282-4286,5.基金项目
国家自然科学基金资助(30971039):《Mash1通路在NGF诱导神经干细胞向胆碱能神经元定向分化中的调控作用及机制》 （30971039）
黑龙江省自然科学基金资助(D2007-02):《Noggin基因修饰神经干细胞移植治疗局灶性脑缺血的实验研究》 （D2007-02）
黑龙江省自然科学基金资助(D200617):神经干细胞移植治疗缺血性脑血管病及移植环境优化的实验研究. （D200617）
