临床口腔医学杂志2011，Vol.27Issue(5)：262-265,4.DOI:10.3969/j.issn.1003-1634.2011.05.002
三种根面洁治方法对人牙周膜细胞再附着和增殖的影响
Three root scaling methods affected human periodontal ligament cell adhesion and proliferation.
周丽华 1刘亚君 1谈珺 1陈芳 1王新文 1王勤涛1
作者信息
- 1. 第四军医大学口腔医学院,陕西,西安,710032
- 折叠
摘要
Abstract
Objective:The teeth affected by periodontal disease were treated by different therapy method to evaluate the influence of root surface roughness and periodontal ligament cell adhesion and proliferation. Method: 24 extracted teeth which affected by periodontal disease were sorted into 3 groups:Gracey treatment group,ultrasonic treatment group and Vector treatment group. Each treatment group were choose 2 root specimens to observe surface roughness by atomic force microscope: 2 root specimens to observe root surface morphology change by scanning electron microscope. Other 12 root specimens were incubate with periodontal ligament cell, 1,3,5 day after incubating,and cell's adhesion was observed by scanning electron microscope and DAPI dyeing was used for cell counting. Result: The roughness of Vector treatment group were lower than Gracey treatment group slightly, but significantly lower than ultrasonic treatment group;The cell's attachment situation of Vector treatment group were similar to Gracey treatment group 1 day after incubate, With the inoculation time extend,the cell's number of Vector treatment group and Gracey treatment group were significantly higher than ultrasonic treatment group. Conclusion: Vector treatment group can get the similar root surface morphology of Gracey, compare with ultrasonic treatment group, Vector is more conducive to attachment of periodontal ligament fibroblasts.关键词
Vector治疗系统/表面粗糙度/细胞附着/细胞增殖Key words
Vector: roughness: cell adhesion/ cell proliferation分类
医药卫生引用本文复制引用
周丽华,刘亚君,谈珺,陈芳,王新文,王勤涛..三种根面洁治方法对人牙周膜细胞再附着和增殖的影响[J].临床口腔医学杂志,2011,27(5):262-265,4.
