中国中医药信息杂志2011，Vol.18Issue(6)：37-39,3.DOI:10.3969/j.issn.1005-5304.2011.06.016
加味丹参饮对血瘀证兔心肌缺血再灌注损伤的影响
Effects of Jiawei Danshen Decoction on Myocardial Ischemia Reperfusion Injury in Rabbits with Blood Stasis Syndrome
摘要
Abstract
Objective To observe the effect of Jiawei Danshen Decoction on myocardial ischemia reperfusion injury (IRI) in blood stasis syndrome (BBS) rabbits, and investigate its mechanism.Method Sixty rabbits were randomly divided into 6 groups with 10 rabbits each: control group, BSS group, IRI group, precondition group, Danshen group and Jiawei Danshen Decoction group.BBS model of IRI was established and interfered by Jiawei Danshen Decoction.Myocardial enzyme (LDH and CK-MB) were detected by automatic biochemistry analysor.The level of cardiomyocyte apoptosis was evaluated by method of terminal deoxynucleotidy transferase mediated nick end labeling (TUNEL), and ultrastructure of myocardial cell was observed by electron microscope.Result Compared with the IRI group, both the contents of LDH and CK-MB were significantly decreased, and cardiomyocyte apoptosis was depressed significanfiy in Jiawei Danshen Decoction group (P ＜0.01 or P ＜0.05).Jiawei Danshen Decoction improved ultrastructure of cardiomyocyte.Conclusion Jiawei Danshen Decoction can inhibit cardiomyocyte apoptosis and the leak of myocardial enzyme, and improve ultrastructure of cardiomyocyte during myocardial IRI,which might be one of the mechanisms of myocardial protection.关键词
血瘀证/心肌缺血再灌注损伤/加味丹参饮/兔Key words
blood stasis syndrome/ myocardial ischemia reperfusion injury/ Jiawei Danshen Decoction/rabbits分类
医药卫生引用本文复制引用
李鑫辉,黄政德,葛金文..加味丹参饮对血瘀证兔心肌缺血再灌注损伤的影响[J].中国中医药信息杂志,2011,18(6):37-39,3.基金项目
国家自然科学基金(30973750) （30973750）
湖南省科技计划项目(2008FJ3070) （2008FJ3070）
湖南省教育厅项目(09A071) （09A071）
