南方农业学报2011，Vol.42Issue(3)：240-245,6.
解磷菌株黑曲霉PSFM发酵条件优化研究
Optimization of fermentation condition for phosphate-solubilizing fungi (Aspergillus niger)
摘要
Abstract
[Ohjective]The present experiment was aimed to study the effects of different ingredients and cultural conditions on phosphate solubilization capacity of Aspergillus niger strain PSFM (isolated from soil) for obtaining best fermentation conditions. [Method]The treatments included different media (inorganic and organic P, SP, NBRIY , NBRIP and NBRIYP) , C (xylose, glucose, lactose, sugar, maltose, galactose, sorbose, mannose, fructose, soluble starch, thamnose ,glycerin) and N (NaNO3, urea, yeast extract, NH4Cl, Mg(NO3)2, (NH4)2SO4, tryptophan, ammonium acetate, heef extract.pancreas-petone, glycine, tyrosine, alanine and arginine) sources. micro- and macronutrients (NaCl. KCl, MgSO4-7H2O,FeSO4-7H2O and MnSO4-7H2O) , fermentation time (0-10 days) , temperature (20-40℃) , initial pH (3.0-11.0) , inoculation quantity (1-11％) . and rotation speed of incubator shaker (120-300 rpm). The orthogonal tests were performed to optimizethe fermentation conditions of phosphate-solubilizing fungi. The absorbance of fermentation liquid was determined to calculate the soluhilized phosphate content. [Result]The soluhilized phosphate content in inorganic liquid medium was highest (526.42 mg/L) at 6 days after culturing in diff'erent media compared to the other mediums. Out of 12 carbon sources, the hest growth of PSFM strain was recorded in xylose followed by glucose, while it was least in medium containing thamnose and glycerin. Out of all the N sources, (NH4)2SO4 showed significantly higher solubilized phosphate content (990.31 mg/L) compared to the other N sources. With the increase of NaCl, KCl, MgSO4·7H2O , FeSO4·7H2O and MnSO4·7H2O concentration in morganic phosphorus medium, the phosphate solubilization capacity of PSFM strain increased initially, followed by a decrease , and it reached the highest at their 0.03 , 0.01 , 0.03 , 0.001 and 0.001％ concentration,respectively. The phosphate solubilization content of PSFM strain was recorded highest as 599.24, 528.23 , 603.69, 530.57 and 731.48 mg/L in 6 days of fermentation. 28℃ temperature , 6.0 0f initial pH, 5％ inoculation quantity and 300 rpm rotation speed , respectively. [ Conclusion ]The results revealed that inorganic phosphorus medium containing 1.0％ xylose (carbon source ) . 0.10％ (NH4)2SO4 (nitrogen source) , 0.1 , 0.7 , 0.7, 0.01 , and 0.01 g/L concentrations of' inorganic salts,viz., NaCl, KCl, MgSO4-7H2O, FeSO4·7H2O and MnSO4·7H2O, respectively, was the best when 5％ Aspergillus niger strain PSFM was cultured f'or 6 days at 28℃ temperature , initial pH 6.0 and 300 rpm rotation speed.关键词
解磷菌/黑曲霉/发酵培养基/发酵条件/优化/正交试验Key words
phosphate-solubilizing fungi/ Aspergillus niger/ f'ermentation medium/ f'ermentation condition/ optimization / orthogonal experiment分类
农业科技引用本文复制引用
梁艳琼,雷照鸣,贺春萍,郑肖兰,郑服丛..解磷菌株黑曲霉PSFM发酵条件优化研究[J].南方农业学报,2011,42(3):240-245,6.基金项目
国家天然橡胶产业技术体系病虫害防控专家岗位项目(nycytx-34-GW2-4-3) （nycytx-34-GW2-4-3）
中央级公益性科研院所基本科研业务费专项项目(2009hzs1J015,2009hzs1J016) （2009hzs1J015,2009hzs1J016）
公益性行业(农业)科研专项项目(nyhyzx07-033-2-3) （农业）
