湖南农业大学学报（自然科学版）2011，Vol.37Issue(3)：237-242,6.DOI:10.3724/SP.J.1238.2011.00237
与棉花无蜜腺基因紧密连锁SSR标记的筛选及SCAR标记的转化
Screening of a SSR marker tightly linked to nectariless gene in cotton and the SCAR marker conversion
摘要
Abstract
A DNA marker linked to the nectariless gene in cotton was explored based on the population of F1 and F2 of 97014×TM-1 by bulk segregant analysis (BSA) and simple sequence repeats(SSRs) technique. A total of 203 SSR primers were screened and a SSR marker(BNL1673) closely linked to nectariless gene(1.33 cM) was identified and then sequenced.Two pairs of specific PCR primers were designed, and the products of SCAR(sequence characterized amplified region)-PCR indicated that only one pair of primers could amplify polymorphic bands between nectary and nectariless traits .The specific pair of primers was chosen to amplify each individual in the F1 and F2 population, and the result showed that the segregation pattern of this SCAR marker for nectariless gene was the same as that of the SSR marker, thus these primers could use as the specific primer of SCAR marker for identifying nectariless gene.关键词
棉花/无蜜腺性状/分子标记/转化Key words
cotton/ nectariless trait/ molecular markers/ conversion分类
农业科技引用本文复制引用
李瑞莲,朱四元,陈金湘,张德胜,刘爱玉,刘海荷..与棉花无蜜腺基因紧密连锁SSR标记的筛选及SCAR标记的转化[J].湖南农业大学学报（自然科学版）,2011,37(3):237-242,6.基金项目
湖南省自然科学基金项目(08JJ3079) （08JJ3079）
