湖南农业大学学报(自然科学版)2011,Vol.37Issue(3):243-247,5.DOI:10.3724/SP.J.1238.2011.00243
苎麻多胚苗遗传多样性的SRAP标记分析
Application of molecular marker SRAP on analysis of genetic diversity of polyembryonic ramie seedlings
摘要
Abstract
To analyze the genetic diversity of polyembryonic ramie, 21 pairs of twin ramie seedlings, a bunch of tri-embryony seedlings and one normal seedling were screened out from the F1 progenies of Jingxi qing and Zhongzhu No.1, and SRAP(sequence related amplified polymorphism)markers were applied in analyzing the genomes of polyembryonic ramie and their parents.The results showed that a total of 159 polymorphic bands were detected with an average of 6.8 polymorphic bands per primer pair.The genetic similarities of 48 materials ranged from 0.360 to 0.876.UPGMA cluster analysis showed that 48 materials were classified into four cluster groups with genetic similarity of 0.51 and group A was further classified into Ⅰ, Ⅱ and Ⅲ sub-groups with genetic similarity of 0.586.There was no material showed total consistency in polymorphic bands with another, so the individuals had nothing to do with apomixis.关键词
苎麻/多胚苗/相关序列扩增多态性(SRAP)/遗传多样性Key words
Boehmeria nivea/ polyembryony/ sequence related amplified polymorphism(SRAP)/ genetic diversity分类
农业科技引用本文复制引用
温岚,喻春明,王延周,陈平,陈继康,谭龙涛,熊和平..苎麻多胚苗遗传多样性的SRAP标记分析[J].湖南农业大学学报(自然科学版),2011,37(3):243-247,5.基金项目
农业部公益性行业(农业)科研专项(nyhyzx07-018) (农业)
国家麻类产业技术体系苎麻育种岗位(CARS-19-E02) (CARS-19-E02)