湖南农业大学学报（自然科学版）2011，Vol.37Issue(3)：267-270,4.DOI:10.3724/SP.J.1238.2011.00267
茶氨酸生物合成基因工程菌的构建及重组酶的表达
Construction of genetically engineered Escherichia coli strain for the biosynthesis of theanine
摘要
Abstract
The γ-G1utamyltranspeptidase gene from Escherichia coli DH5α was cloned in pET-32α vector and expressed in Escherichia coli BL21.When the recombinant strain grew at pH 7 to obtain the OD6oonm of 1.5 and induced with 0.1 mmol/L IPTG at 20 ℃ for 6 h, the activity of γ-Glutamyltranspeptidase was (4.41±0.17) U/mL, about 18.4 times than that of Escherichia coli DH5α.Under the catalysis of cells of the genetically engineered strain and under the pH 10, thetemperature of 20 ℃ and the incubation time of 6 h, the yield of theanine from L-Gin (200 mmol/L) and ethylamine (1.5 mol/L) was 12.6 mg/mL, and the rate of conversion from L-glutamine to theanine was 41.05％.关键词
茶氨酸/工程菌/γ-谷氨酰转肽酶/重组质粒/重组酶Key words
theanine/ engineered strain/ γ-G1utamyltranspeptidase/ recombinant plasmid/ recombinase分类
农业科技引用本文复制引用
李勤,黄建安,李娟,刘仲华..茶氨酸生物合成基因工程菌的构建及重组酶的表达[J].湖南农业大学学报（自然科学版）,2011,37(3):267-270,4.基金项目
国家自然科学基金(30871572) （30871572）
国家"973"计划前期研究专项(2008CB117007) （2008CB117007）
湖南农业大学人才科学基金(07WD22) （07WD22）
