生物加工过程2011，Vol.9Issue(3)：11-16,6.DOI:10.3969/j.issn.1672-3678.2011.03.003
原生质体紫外诱变技术选育1,3-丙二醇高产菌株
Screening of 1,3-propanediol high yield strain by UV mutagenesis of protoplast
摘要
Abstract
High glycerol and 1,3-propanediol tolerance strain of Klebsiella pneumoniae was screened by ultraviolet mutagenesis of protoplast. Filtering by millipore simplified the enzymolysis process and enhance the regeneration rate and formation rate during the process of protoplast preparation. After protoplast mutagenesis, aimed at the tolerance of high concentrations of glycerol and 1,3-propanediol and high productivity of acid, three high yield mutants, Kp-1, Kp-4 and Kp-5, were obtained. In fed-batch fermentation, the volumetric productivities of above strains were 70.24 g/L, 65.21 g/L, and 75.51 g/L,respectively, increased by 25.92％, 16.91％ and 35.37％ compared with the wild type strain.关键词
1,3-丙二醇/肺炎克雷伯氏杆菌/甘油/原生质体/诱变Key words
1,3-propanediol/ Klebsiella pneumoniae/ glycerol/ protoplast/ mutagenesis分类
生物科学引用本文复制引用
卢圣国,熊骏,祝扬扬,郭凡财,孟庆雄..原生质体紫外诱变技术选育1,3-丙二醇高产菌株[J].生物加工过程,2011,9(3):11-16,6.基金项目
国家高技术研究发展计划(863计划)资助项目(2009CB724701) （863计划）
