生物加工过程2011,Vol.9Issue(3):37-40,4.DOI:10.3969/j.issn.1672-3678.2011.03.008
海洋细菌Agarivorans albus NBRC102603琼胶酶的分离纯化
Isolation and purification of agarase from marine bacteria Agarivorans albus NBRC102603
摘要
Abstract
Agarases A and B were separated and purified by salting-out, molecular sieve, and ion exchange from fermentation broth of marine bacteria Agarivorans albus NBRC102603.The obtained enzyme agarase A was purified 17.6 folds with specific activity of 881.82 U/mg, while agarase B was purified 16.64 folds with specific activity of 838.2 U/mg.The enzymes were identified to be homogeneous by SDS-PAGE.The molecular masses were 8.36 × 104 and 3.68 × 104, respectively.关键词
海洋细菌/琼胶酶/分离/纯化Key words
marine bacteria/ agarase/ separation/ purification分类
生物科学引用本文复制引用
朱启忠,朱慧文,孙艳娜,周晓龙,郝梅,郭振博..海洋细菌Agarivorans albus NBRC102603琼胶酶的分离纯化[J].生物加工过程,2011,9(3):37-40,4.基金项目
国家创新性基金项目(101042239) (101042239)
山东大学科技立项重点项目专项基金(A10010) (A10010)