医药导报2011，Vol.30Issue(8)：1069-1071,3.DOI:10.3870/yydb.2011.08.036
槲皮素固体脂质纳米粒的制备与含量测定
Preparation and Content Determination of Quercetin Solid Lipid Nanoparticles
摘要
Abstract
Objective To establish a preparatian process and content determination method for quercetin solid lipid nanoparticles( QUE-SLNs ). Methods The oil phase for preparing QUE-SLNs was mainly made of Glyceryl behenate ( ATO ),soy lecithin and cholesterol, and the aqueous phase was made of poloxamer, PEG-DPSE and Tween 80. The temperature for emulsion was set at 75 ℃ , for solification from ice to water was at 2 ℃ ,and the agitator for emulsion was 600 r · min-1. The quercetin was determinated by a high-velocity centrifugation and HPLC. The Diamonsil C18( 250 mm×4.6 mm,5μm ) was used, methanol-acetic acid ( 55∶ 45 ) was taken as mobile phase at 1.0 mL · min -1 , column temperature was 30 ℃ and the sample was loaded with 20 μL. Results QUE-SLNs were stable and presented as linear relation at the range of 20.0 ～ 200.0 μg · mL 1 ,A= 1. 343 5Cl. 490 4( r= 0. 999 6 ). The average recovery was 97.7％ and RSD was 1.09％ ( n = 6 ). Conclusion Peparation process for QUE-LCL is stable and content determination is simple, reliable, accuracy, and feasibility.关键词
槲皮素/固体脂质纳米粒/含量测定Key words
Quercetin/ Solid lipid nanoparticles/ Centent determination分类
医药卫生引用本文复制引用
常明泉,孙运娥,蒋安荣,王刚..槲皮素固体脂质纳米粒的制备与含量测定[J].医药导报,2011,30(8):1069-1071,3.基金项目
湖北省教育厅优秀中青年基金资助项目(基金编号:Q201002110) （基金编号:Q201002110）
