中国临床药理学与治疗学2011，Vol.16Issue(4)：361-365,5.
人参皂苷Rg1对人胃癌BGC-823的抑制作用研究
Inhibitory effect of ginsenoside Rgl on BGC-823 human gastric cancer cell line
摘要
Abstract
AIM: To observe the influences of Ginsenoside Rgl on the activity, proliferation,the expression of Bax-2 and caspase-3 and morphology of BGC-823 gastric cancer cell line, and investigate its pharmacological mechanisms.METHODS: Cells which were stayed in logarithmic growth were used for the experiments, the cells were interfered with different concentration of Ginsenoside Rgl, the methods of growth curve, M'IT assay, protein content assay Realtime PCR and morphological observation were used to investigate the effects of Ginsenoside Rgl on BGC-823 cells. RESULTS: Ginsenoside Rgl had potent inhibitory effect on BGC-823 cell line in a dose-dependent and time-dependent manner. Ginsenoside Rgl had significant cytotoxic effect to BGC-823 cells, and its half maximal inhibitory concentration (IC50) is 29.56 mg/L. Ginsenoside Rgl decreased the amount of protein in BGC-823 cells, improved the expression of Bax-2、caspase-3, and caused the shrinkage of cells, the cytoplasm decreased, and the color was salmon pink; the chromatin condensed and the color was intense violet; the cellular nucleuses were condensed or broken into round granules. CONCLUSION: Ginsenoside Rgl obviously inhibited the cell proliferation, decreased its activity, showed conspicuous anti-tumor effect on BGC-823 cells. The anti-tumor effect may be related to the inhibition effect to tumor cell protein synthesis, and promote the apoptosis of BGC-823 cells.关键词
人参皂苷Rg1/胃癌/BGC-823细胞株/细胞增殖/细胞凋亡Key words
Ginsenoside Rgl/ Gastric cancer/ BGC-823/ Cell proliferation/ Apoptosis分类
医药卫生引用本文复制引用
赵保胜,刘洋,徐暾海..人参皂苷Rg1对人胃癌BGC-823的抑制作用研究[J].中国临床药理学与治疗学,2011,16(4):361-365,5.基金项目
教育部科学技术研究重点项目(108132) （108132）
北京市教育委员会共建项目 （）
