中国临床药理学与治疗学2011，Vol.16Issue(4)：374-379,6.
甘油醛-3-磷酸脱氢酶核转位与高糖应激诱导的H9c2细胞凋亡密切相关
High glucose-induced apoptosis of H9c2 cells is closely related to GAPDH nuclear translocation
孙传波 1高景红 1胡亮 1张竞文 1魏立 1李庆平1
作者信息
- 1. 南京医科大学基础医学院药理学系,江苏省心血管病分子干预重点实验室,南京210029,江苏
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摘要
Abstract
AIM: To investigate whether high glucose induces GAPDH nuclear translocation and whether the GAPDH nuclear translocation is related to high glucose-induced apoptosis of H9c2 cells.METHODS: H9c2 cells were cultured for 48 h in high glucose condition, and then cells were collected for apoptosis determination.The generation of reactive oxygen species (ROS) was determined.The expression of GAPDH and iNOS was measured by western blotting.The translocation of GAPDH was determined by immunofluorescence.RESULTS:High glucose induced apoptosis of H9c2 cells.High glucose induced oxidative stress in H9c2 cells and translocation of GAPDH to nucleus.Rotenone and iNOS inhibitor 1400W protected H9c2 cells from death by inhibiting GAPDH translocation to nucleus.CONCLUSION: High glucose induces GAPDH translocation from cytoplasm to nucleus.The inhibition of high glucose-induced apoptosis of H9c2 cells is closely related to GAPDH nuclear translocation.关键词
H9c2细胞/高糖/细胞凋亡/甘油醛-3-磷酸脱氢酶核转位/一氧化氮合酶Key words
H9c2 cells/ High glucose/ Cell apoptosis/ GAPDH translocation/ Nitric oxide synthase分类
医药卫生引用本文复制引用
孙传波,高景红,胡亮,张竞文,魏立,李庆平..甘油醛-3-磷酸脱氢酶核转位与高糖应激诱导的H9c2细胞凋亡密切相关[J].中国临床药理学与治疗学,2011,16(4):374-379,6.
