安徽农业科学2011，Vol.39Issue(14)：8364-8366,3.
大孔吸附树脂纯化甘草黄酮类化合物的研究
Purification of Flavonoids from RADIX RHIZOMA GLYCYRRHIZAE by Macroporous Resins
易运红 1吴功庆 1曾富兰 1刘意1
作者信息
- 1. 广东药学院中山校区,广东中山,528458
- 折叠
摘要
Abstract
[ Objective ] To optimize the purification conditions of flavonoids from RADIX RHIZOMA GLYCYRRHIZAE by macroporous resins.[ Method] 4 kinds of macroporous resins including ADS-7 ,D101, HPD-300, HPD-910 and AB-8 were adopted to ab~rb flavonoids from RADIX RHIZOMA GLYCYRRHIZAE. Besides,effects of factors affecting the adsorption properties such as sample concentration, ethanol concentration and ethanol volume were also investigated. [ Result] ADS-7 was provided with higher absorption rate and the elution rate of flavonoids from RADIX RHIZOMA GLYCYRRHIZAE ,the optimum effect of which were obtained with sample concentration at 1.2 mg/ml using 5BV of 0.70 ethanol as eluent. After purification,the content and refined rate of flavonoids in powder were 47. 1％ and 211.2％ ,respectively. [ Conclusion] ADS-7 resin had strong adsorption and purification effects,which would provide reference basis for purifying flavonoids from RADIX RHIZOMA GLYCYRRHIZAE.关键词
甘草(RADIX RHIZOMA GLYCYRRHIZAE)/黄酮类化合物/大孔吸附树脂Key words
RADIX RHIZOMA GLYCYRRHIZAE/ Flavonoids/ Macroporous resins分类
农业科技引用本文复制引用
易运红,吴功庆,曾富兰,刘意..大孔吸附树脂纯化甘草黄酮类化合物的研究[J].安徽农业科学,2011,39(14):8364-8366,3.
