华北农学报2011，Vol.26Issue(2)：7-12,6.
一株抗铜根瘤菌的分离鉴定及其16S rDNA序列分析
Isolation,Identification and 16S rDNA Sequences Analysis of a Rhizobium Strain Resistant to Copper
摘要
Abstract
In recent years ,with the development of mining, smelting and processing of copper industry, soil copper pollution is becoming increasingly serious. Thus it is imperative to restore the soil copper contamination. In this study, in order to develop collaborative restoration activity of microorganisms and plants in the copper-contaminated soils of heavy metals tailing area, an excellent Rhizobium strain resistance to copper was screened from mining tailings Fengxlan county,Shaanxi province. The strain,named CCNWSX0020,resisted to 3.0 mmol/L Cu2+ on YMA agar plate and to 1.4 mmol/L Cu2+ in TY liquid medium, so it is an important strain using to study the resistant copper mechanisms of microbe. It was preliminarily identified as a strain of Sinorhizobium meliloti, based on its physiological and biochemical characteristics analysis, including G + C mol％ determination, DNA homology hybridization and 16S rDNA sequence similarity comparison. The similarity between CCNWSX0020 and S. meliloti LMG6133 ( GenBank accession number X67222) was 99.7％. Because of Rhizobia with the unique biology status that is being able to form symbiont with legume,which can provide a good genetic base for the further construction of a biology system being tolerant to heavy metals, and as a result,having potential ecological and social benefits.关键词
重金属/铜/根瘤菌/16S rDNAKey words
Heavy metals/ Copper/ Rhizobia/ 16S rDNA分类
生物科学引用本文复制引用
樊连梅,李哲斐,韦革宏..一株抗铜根瘤菌的分离鉴定及其16S rDNA序列分析[J].华北农学报,2011,26(2):7-12,6.基金项目
国家自然科学基金(31070444 （）
30970003 （）
30900215) （）
