华北农学报2011，Vol.26Issue(2)：26-29,4.
棉花GhCAD1基因结构及表达分析
Structural and Expression Analysis of GhCAD1 Gene from Gossypium hirsuturm L.
摘要
Abstract
According to the eDNA sequence from cotton GhCAD1 gene, primers were designed. PCR method was used to clone GhCAD1 gene and the expression of GhCAD1 gene in different tissues of cotton was analyzed by semiquantitative RT-PCR. A length of 2 898 bp sequence from genomic DNA of GhCAD1 was cloned by PCR. The genomic DNA of GhCAD1 contains five exons and four introns. Analysis of these introns indicated that they are rich in AT. Furthermore, a SBF-1 factor binding site was identified in the first intron of GhCAD1. Semi-quantitative RT-PCR analysis revealed that GhCAD1 can be expressed in different kinds of cotton tissues,and its mRNA accumulated most abundantly in leaf. GhCAD1 expression in cotton tissues was leaf ＞ boll shell ＞ bract ＞ petal ＞ root ＞ cotyledon.关键词
棉花/GhCAD1/内含子/表达分析Key words
Gossypium hirsuturm L./ GhCAD1/ Intron/ Expression analysis分类
生物科学引用本文复制引用
倪志勇,吕淑萍,李波,范玲..棉花GhCAD1基因结构及表达分析[J].华北农学报,2011,26(2):26-29,4.基金项目
农业部转基因重大专项(2009ZX08005-011B) （2009ZX08005-011B）
国家自然科学基金项目(31060173) （31060173）
国家"863"项目(2006AA10Z184) （2006AA10Z184）
国家自然科学基金(30660088) （30660088）
新疆自治区高技术研究发展计划(200611101) （200611101）
