华北农学报2011，Vol.26Issue(2)：70-75,6.
呼和浩特市区牛种布鲁菌bp26和omp10的克隆
The Cloning of bp26 and omplO Genes of Brucella Abortus in Huhhot
摘要
Abstract
To clear genes homology on gene of the Brucella outer membrane protein BP26 and OMP10 genes among Brucella of testing or reference and Genbank strains, clone and Comparison for their DNA sequences . We designed and synthesized a specific primers with Brucella bp26 and omplO in Genbank. The genomic DNA of bp26 and omplO of testing or reference Brucella strains was extracted from each sample,PCR amplification was then carried out and thus obtaining bp26 and omplO. The PCR product was inserted into pMD18-T vector and sequenced. The plasmids were transformed into E. coli DH5α. Extracted E. coli plasmid DNA, PCR detected and sequenced. Sequencing showed that bp26 was 995 bp in length,including a 753 bp open reading of the complete framework . Sequence comparison reyealed that homology of bp26 among the testing strains was 100％ ,homology of bp26 of the testing and the reference M5 or S2 strains were 100％ or 99.9％ , homology of bp26 of the testing strains and the reference S19 or 870 sequences was 99.9％ or 100％. omplO was 531 bp containing a 396 bp open reading of the complete framework, homology of omplO among the testing strains was 100％. homology of omplO of the testing and the reference M5 or S2 strains were 100％. homology of omplO of the testing and the reference 544 sequence were 99.7％. There are bp26 and omplO gene homology between the various types in more than 99％. They is highly remain stable.关键词
布鲁菌/bp26/omp10/同源/分析Key words
Brucella/ bp26/ omplO/ Homology/ Analysis分类
农业科技引用本文复制引用
王军,王瑞,杨莲茹,郭志亮,于翠玲,申之义..呼和浩特市区牛种布鲁菌bp26和omp10的克隆[J].华北农学报,2011,26(2):70-75,6.基金项目
内蒙古自治区自然科学基金资助项目(200508010412) （200508010412）
