华北农学报2011，Vol.26Issue(2)：128-132,5.
华仁杏ISSR-PCR反应体系的正交优化分析
Optimization of ISSR-PCR System of Armeniaca cathayana Through Orthogonal Design Method
摘要
Abstract
Armeniaca cathayana D. L. Fu et al. is a new species described in 2010 and has high values for its big and not bitter kernels. In order to research its germ plasma and genetic diversity based on ISSR technique, the orthogonal design of L16(45 )was used to optimize the ISSR-PCR amplification system of A. cathayana in five major factors.Based on range analysis and variance analysis,the best ISSR-PCR reaction system(25 μL)was established:lO × PCR Buffer without MgCl2 2.5 μL, MgCl2 2.0 mmol/L, Taq DNA Polymerase 2.0 U ,dNTP MiX 100 μmol/L, ISSR Primer 0.2 μmol/L,DNA 2.4 ng/μL.关键词
华仁杏/杏属/ISSR-PCR反应体系/正交设计Key words
Armeniaca cathayana /Armeniaca Scop/ ISSR-PCR amplification system/ Orthogonal design分类
农业科技引用本文复制引用
刘梦培,傅大立,李芳东,傅建敏,田敏..华仁杏ISSR-PCR反应体系的正交优化分析[J].华北农学报,2011,26(2):128-132,5.基金项目
国家"十一五"科技支撑项目资助(200BAD01A1703-2) （200BAD01A1703-2）
