重庆医学2011，Vol.40Issue(22)：2208-2210,封4,4.DOI:10.3969/j.issn.1671-8348.2011.22.009
RNA干扰Axin2对大鼠骨髓基质干细胞的成骨分化表达的影响
Effect of RNAi of Axin2 on osteogenic differentiation and mRNA expression of rat bone marrow stromal cells
摘要
Abstract
Objective To study the effect of Axin2 on osteogenic differentiation of bone marrow stromal cells(BMSCs) of rats by RNA interference.Methods BMSCs derived from the tibia and femur of 6-week old female SD rats were cultured in vitro.After the second passage,the cells in experimental group were transfected with the RNAi plasmid targeting on Axin2 and the cells in control group were transfected with the negative control plasmid.48 hours later, all the cells were treated with osteogenic inductive DMEM.Von Kossa staining was applied at 28 days later to assess extracellular matrix mineralization.Real time quantitative-PCR was performed to evaluate the expressions of β-catenin, OCN,frizzled-2 ,Lef-1 and Wnt5a mRNA at day 7 and day 14 after osteogenic induction.Results Cells in control group were differentiated into osteoblast lineage,as shown by mineralization of extracellular matrix but not in experimental group.Compared with control group,the expressions of β-catenin and frizzled-2 mRNA were significantly lower and OCN,Lef-1 and wnt5a mRNA expression were significantly higher in experimental group at day 7.The expressions of frizzled2,Lef-1 and Wnt5a mRNA in experimental group were significantly higher than those in control group at day 14 (P＜0.05).Conclusion When RNAi targeting the mRNA of Axin2 is performed, the ability of extracellular matrix to mineralize decreases in the late process of the osteogenic differentiation of BMSCs.关键词
成骨细胞/RNA干扰/干细胞/Wnt信号转导途径Key words
osteoblasts / RNA interference/ stem cells / wnt signaling引用本文复制引用
张磊,张柳,高建清,田发明,韩大成,牛军强,刘晓宁..RNA干扰Axin2对大鼠骨髓基质干细胞的成骨分化表达的影响[J].重庆医学,2011,40(22):2208-2210,封4,4.基金项目
河北省自然科学基金资助项目(C200600580). （C200600580）
