现代妇产科进展2011，Vol.20Issue(5)：372-376,5.
青岛地区宫颈病变组织中HPV16 URR和E6基因突变分析
Analysis of variants of HPV16 URR and E6 among Qingdao women with cervical lesions
赵成琳 1王言奎 1罗兵 2王玉婷 1彭丽娜3
作者信息
- 1. 青岛大学医学院附属医院妇产科,青岛266003
- 2. 青岛大学医学院微生物学教研室
- 3. 聊城市人民医院妇产科
- 折叠
摘要
Abstract
Objective: The study was performed to investigate genetic vanants of the HPV16 URR and E6 genes derived from Qingdao women with different stages of cervical lesions,and to assess the association between the sequence variants and the cervical lesions.Methods : We use the technology of polymerase chain reaction( PCR) to detect 120 cases of cervical cancer,254 cases with cewical intraepithelial neoplasia( CIN) . Then we selected samples with HPV16 positive,and amplifies the HPV16 URR and E6 genes. The products of PCR were purified and sequenced, and then were compared with the standard strain. Results : There were 19 kinds of mutations in the URR gene. The mutation at nt 752lfrom G to A was most frequent.A remarkable finding was the co-nutations including nt 24 ,nt 7730 and nt 7842. The most frequent sequence variation of E6 was nt 178.which was 61. 11％ (44/72) ,62. 00％ (31/50) and 50. 00％ (16/32) in cervical cancer, CIN Ⅲ and CIN Ⅱ . The mutation of L83V was only found in 6 cases. In Qingdao area, two major branches of HPV16 were type AS ( 59. 09％ ) , followed by prototype(33. 12％ ) . Conclusion : The mutational hot spots of HPV16 URR are nt7521 and a co-mutation including nt24, nt7830 and nt7842. The hot spot of E6 is nt178. These variants may be associated with the developing cervical cancer. In Qingdao area,two major branches of HPV16 are type AS and prototype.关键词
宫颈肿瘤/宫颈疾病/人乳头瘤病毒16型/URR/E6/突变Key words
Cervical neoplasms/ Cervical diseases/ Human papillimovirs 16/ URR/E6 / Variant分类
医药卫生引用本文复制引用
赵成琳,王言奎,罗兵,王玉婷,彭丽娜..青岛地区宫颈病变组织中HPV16 URR和E6基因突变分析[J].现代妇产科进展,2011,20(5):372-376,5.
