中华临床免疫和变态反应杂志2011，Vol.5Issue(1)：17-23,7.
维生素D受体Bsm I基因多态性及VDR mRNA表达与系统性红斑狼疮的相关性
Association of Vitamin D Receptor Gene Bsm I Polymorphism and mRNA Expression with Systemic Lupus Erythematosus
摘要
Abstract
Objective To investigate the association of vitamin D receptor (VDR) gene Bsm Ⅰ polymorphism with systemic lupus erythematosus (SLE) and the influence to VDR mRNA in SLE in Chinese Han patients.Methods Genomic DNA from 242 Chinese SLE patients and 162 sex and ethnically matched controls were typed for VDR Bsm Ⅰ polymorphism by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Clinical characteristics were analyzed among different Bsm Ⅰ genotypes. The expression of VDR mRNA from 48 Chinese SLE patients and 38 sex and ethnically matched controls were detected with real-time polymerase chain reaction (RT-PCR). Results There was no significant difference between frequencies of allelic gene B and b in SLE and controls (P=0.166). But SLE patients carrying allelic gene B (BB and Bb genotypes) which were more involved in renal disorder when compared with patients carrying allelic gene B negative (bb genotype, P=0.044). The expression of VDR mRNA in 48 SLE was down-regulating, while the⊿Ct value of SLE was higher than controls (9.26±2.37 vs 7.82±3.05,P=0.026) (the higher of ⊿Ct value, the lower of expression quantity). The ⊿Ct value of VDR mRNA in SLE carrying B allele was higher than that in B allele negative SLE (9.53±0.53 vs 7.77±4.30, t = 2.502,P=0.016). Conclusion There is no association between the VDR gene Bsm Ⅰ polymorphism and systemic lupus erythematosus in the Han population of southern China, but the patients carrying B allele are more involved in renal disorder which may be due to carrying B allele down-regulating the expression of VDR mRNA in SLE.关键词
系统性红斑狼疮/维生素D受体/Bsm I/基因多态性Key words
systemic lupus erythematosus/ vitamin D receptor/ Bsm Ⅰ/ gene polymorphism分类
医药卫生引用本文复制引用
罗雄燕,唐中,赵岩,曾小峰,袁国华,武丽君,陈龙,杨明辉,刘宁涛,库尔班江,谢传美,廖涛,史冉庚..维生素D受体Bsm I基因多态性及VDR mRNA表达与系统性红斑狼疮的相关性[J].中华临床免疫和变态反应杂志,2011,5(1):17-23,7.基金项目
卫生部重大疾病研究资助项目(2008BA159B02).川北医学院重点实验室开放基金资助项目(KFJJ(09)-03) （2008BA159B02）
