重庆医学2011，Vol.40Issue(22)：2185-2187,2190,封2,5.DOI:10.3969/j.issn.1671-8348.2011.22.001
不同浓度GDNF诱导神经干细胞向多巴胺能神经元分化研究
Research on the induction of differentiation of neural stem cells with dopaminergic neurons by different concentrations of glial cell line-derived neurotrophic factors
摘要
Abstract
Objective To investigate the effects of glial cell line-derived neurotrophic factor with different concentrations on the differentiation of neural stem cells ( NSCs) into dopamine(DA) neurons in vitro.Methods The primary NSCs were isolated from the whole brains of neonatal rats.0,5,10,20 ng/mL GDNFs were added into the second generation of NSCs to induce DA neurons for 10 days.Tyrosine hydroxylase (TH) mRNA was detected by RT-PCR;NSCs and DA neurons differentiation from NSCs were detected by immunocytochemiscal stain assay.Results All groups could express TH mRNA.The neurospheres neurospheres had the capacity of selfrenewing , expression of nidogen , and could differentiate into multidirections.Specific antigens of neurons , astrocytesand oligodendrocytes were also expressed.Compared with blank control group,GDNF increased the proportion of TH-positive cells(P＜0.05) ,10,20 ng/mL GDNF were significantly higher than that of 5 ng/mL group(P＜0.05) , while no significant difference was found between 10.20 ng/mL GDNF groups(P＞0.05).onclusion The NSCs were isolated from the brains of neonatal rats.GDNF could promote the differentiation of NSCs into DA neurons,and TH positive cell rate changed minimally when GDNF was increased from IO to 20 ng/mL.关键词
细胞分化/胶质细胞源性神经营养因子/神经干细胞/多巴胺能神经元Key words
cell differentiation / glial cell line-derived neurotrophic factor / neural stem cells / dopaminergic neurons引用本文复制引用
陈梅玲,沈岳飞,李清华,刘开祥,曾爱源,林小慧..不同浓度GDNF诱导神经干细胞向多巴胺能神经元分化研究[J].重庆医学,2011,40(22):2185-2187,2190,封2,5.基金项目
广西自然科学基金资助项目(桂科自0832140) （桂科自0832140）
广西卫生厅基金资助项目(Z2010309). （Z2010309）
