农业工程学报2011，Vol.27Issue(4)：370-375,6.DOI:10.3969/j.issn.1002-6819.2011.04.065
菜籽粕植酸提取和分离蛋白的制备
Extraction of phytic acid and preparation of protein isolates from rapeseed meal
摘要
Abstract
Phytic acid and protein are two kinds of valuable components in rapeseed meal. To improve comprehensive utilization in this research, phytic acid was extracted with acetum from double-low coldpressed rapeseed meal, and protein was recovered from the crude extract of phytic acid by membrane separating technology. The meal residue was dried to extract protein, which was then purified by ultrafiltration to obtain high purity product. Extraction conditions of phytic acid were optimized by response surface methodology (RSM) as follows: mass fraction of acetic acid 0.7％,extraction temperature 48℃, liquid-to-solid ratio 10 mL/g, extraction time 1.6 h. Under this condition, extraction yield was 1.865％. Protein recovered and phytic acid loss accounting for rapeseed meal were 3.63％ and 0.395％ respectively in crude extract of phytic acid. Purity of the protein refined by ultrafiltration was between 70％ and 90％, in which the content ofpolyphenols was significantly reduced and no phytic acid and glucosinolates could be detected.关键词
提取/蛋白/优化/菜籽粕/植酸Key words
extraction/ proteins/ optimization/ rapeseed meal/ phytic acid分类
农业科技引用本文复制引用
潘丽军,周俊,姜绍通,孙汉巨,罗水忠,韩智宏..菜籽粕植酸提取和分离蛋白的制备[J].农业工程学报,2011,27(4):370-375,6.基金项目
国家"十一五"科技支撑计划项目:双低油菜制油新技术与系列产品开发及产业化示范(2006BAD05A12) （2006BAD05A12）
