上海海洋大学学报2011，Vol.20Issue(3)：350-357,8.
乐清湾和三沙湾缢蛏群体遗传多样性的微卫星分析
Microsatellite analysis on genetic diversity of Sinonovacula constricta stocks in Yueqing Bay and Sansha Bay
摘要
Abstract
Genetic diversity and genetic differentiation of 18 stocks of Sinonovacula constricta in Yueqing Bay and Sansha Bay, were investigated by using eight microsatellite loci, in order to evaluate the genetic divergence between Yueqing Bay and Sansha Bay, protecting aquatic genetic resources and providing the basic information of genetic breeding in sourceland of Sinonovacula constricta in China.The results of genetic diversity showed that: the average effective allele numbers ( Ne ) of Yueqing Bay and Sansha Bay ranged from 8.146 to 10.457 and from 7.457 to 9.947; the average expected heterozygosities ( He ) varied from 0.872 to 0.909 and from 0.846 to 0.894; the average genetic diversity index of Nei' s ranged from 0.863 to 0.899 and from 0.836 to 0.886, respectively.Those data indicated higher genetic diversity in all of the 18 stocks in Yueqing Bay and Sansha Bay.Pair-wise FST values between stocks of Yueqing Bay and Sansha Bay ranged from 0.000 1 to 0.052 3, which indicated that there was some degree of differentiation among 18 stocks based on FST.The UPGMA phylogenetic trees based on Ds genetic distance suggested that the 18 stocks were divided into two groups, one of which included the stocks of ZYC, ZNY, ZNT, ZQJ, ZHW, ZHS and ZLP in Yueqing Bay whereas the other one consisted of ZWY and ZWG in Yueqing Bay and FZW, FMT, FSD, FSJ,FBZ, FXT, FCC, FSJ and FXW in Sansha Bay.As a result, we can infer that the stocks of ZWY and ZWG in Zhejiang province may come from Fujian Province, reve aling the introduction phenomenon in the culture of Sinonovacula constricta.关键词
缢蛏/乐清湾/三沙湾/微卫星标记/遗传多样性Key words
Sinonovacula constricta/ Yueqing Bay/ Sansha Bay/ microsatellite DNA/ genetic diversity分类
农业科技引用本文复制引用
刘达博,牛东红,冯冰冰,钟玉民,李家乐..乐清湾和三沙湾缢蛏群体遗传多样性的微卫星分析[J].上海海洋大学学报,2011,20(3):350-357,8.基金项目
国家"八六三"计划项目(2006AA10A410) （2006AA10A410）
上海市高校优青科研专项基金(SSC09016) （SSC09016）
上海市高校博士启动基金(A2400090144) （A2400090144）
