中国骨伤2011，Vol.24Issue(5)：387-391,5.DOI:10.3969/j.issn.1003-0034.2011.05.008
补肾强督方对强直性脊柱炎患者外周血单个核细胞产生MMP-9和TIMP-1的影响
Effects of Bushen Qiangdu decoction(补肾强督方)on MMP-9and TIMP-1 produced by peripheral blood mononuclear cells in patients with ankylosing spondylitis
张英泽 1阎小萍 1叶丽亚 2张文健 2娄晋宁2
作者信息
- 1. 中日友好医院中医风湿病科,北京,100029
- 2. 中日友好医院临床医学研究所
- 折叠
摘要
Abstract
Objective: In order to investigate the roles of metalloproteinase in inflammatory bone destruction in ankylosing spondylitis (AS) ,and analyze the mechanism of preventing inflammatory bone destruction of Bushen Qiangdu decoction (BSQDD) in AS cases.Comparisons were made on the expressions of matrix metalloproteinase 9 (MMP-9) and tissue inhibitor of metalloproteinase 1 (TIMP-1) by peripheral blood mononuclear cells (PBMC) between AS patients and healthy controls.The effect of BSQDD was investigated on the expression and of MMP-9 and TIMP-1 produced by PBMC in AS patients.Methods:From March 2005 to March 2006,30 active AS cases of Kidney-asthenia, Du-cold and blood-stasis syndrome were selected as patients group in the China-Japan Friendship Hospital.There are 27 male patients and 3 female patients.The age range is from 16 to 45 ,averaging (30.8±8.8) years.Disease duration is from 0.5 to 10 years.Cases received three-month BSQDD treatment were considered as the treatment group.Twenty healthy persons were included in the control group.Serum and PBMC were separated.The PBMC were stimulated by PHA and PMA,and the supernatant was collected.The mRNA expression of MMP-9 and TIMP-1 in PBMC was analyzed by RT-PCR.The content of MMP-9 and TIMP-1 in serum and culture supernatant of PBMC were detected by ELISA.Results: Compared with health control group, the serum concentration of MMP-9 and TIMP-1 in patients group before treatment increased (P＜0.01 ,P＜0.05) ,but the level of MMP-9 and TIMP-1 in the serum of patients after treatment decreased compared with pre-treatment cases (P＜0.05).Furthermore,compared with health control group,PBMC of patients group before treatment expressed higher levels of MMP-9 and TIMP-1 both on transcript level and at protein level (P＜0.01 ,P＜0.05), and the expression levels of MMP-9 and TIMP-1 in PBMC in patients after treatment both on transcript level and at protein level was down-regulated compared with pre-treatment (P＜0.01 ,P＜0.05).Conclusion:PBMC of AS patients had a higher potential capacity for MMP-9 and TIMP-1.BSQDD possibly prevented inflammatory bone destruction of AS through inhibiting production of MMP-9 and TIMP-1 produced by PBMC.关键词
脊柱炎,强直性/补肾强督方/单个核细胞,外周血/基质金属蛋白酶9/基质金属蛋白酶组织抑制因子1Key words
Spondylitis,ankylosing/ Bushen Qiangdu decoction/ Peripheral blood mononuclear cells/ Matrix metalloproteinase-9/ Tissue Inhibitor of metalloproteinase-1引用本文复制引用
张英泽,阎小萍,叶丽亚,张文健,娄晋宁..补肾强督方对强直性脊柱炎患者外周血单个核细胞产生MMP-9和TIMP-1的影响[J].中国骨伤,2011,24(5):387-391,5.
