解放军医学杂志2011，Vol.36Issue(5)：478-482,5.
miR-451对结肠癌细胞系SW620生物学行为的影响
Effects of miR-451 on the biological behavior of colorectal carcinona cell line SW620
摘要
Abstract
Objective To explore the effects of human rniR-451 on the proliferation, apoptosis and invasive ability of colon cancer cell line SW620. Methods Four groups were designed in the present experiment, i. e. , ceils were transfected with 100nmol/L of miR-451mimics in rniR-451 mimics group, with 100nmol/L of negative control in negative control (NC) group, with Lipo 2000 alone in blank control group, and without transfection of either Lipo2000 or miRNA in control group. The expression of miR-451 was measured by realtime RT-PCR 24h after transfection, and proliferation of cells was examined by using the Cell Counting Kit-8 at respective time of 24, 48,72 hours after transfction. The apoptotic rate was assessed by flow cytometry (FCM) and invasive ability was determined by Transwell assay. The effect of miR-451 mimics on the expression of macrophage migration inhibitory factor (MIF) protein was measured by immunofluorescent and Western blot assay. Results Twenty-four hours after transfection, an increase in expression of miR-451 was noted in miR-451 mimics group (67. 96 ± 13. 33) when compared with NC group, in which the expression of miR-451 was defined as 1 (P＜0. 01). In comparison with the other groups, miR-451 mimics group showed a significant inhibition of proliferation at 48 and 72 hours after transfection (P＜0. 01), but no significant change in apoptotic rate was found at 48 hour after transfection (P＞0. 05). Immunofluorescent assay showed that MIF positive rate of miR-451 mimics group (23. 9％±14. 9％) was decreased when compared with that of NC group (53. 5％±14. 1％), blank transfection group (45. 2％ ± 19. 8％) and blank control group (59. 3％ ±22. 2％) (F＝ 7. 356, P＜0. 01).Transwell assay illustrated that the invasive ability in miR-451 mimics group (37. 4±6. 1 invasive cells per field) was lower than that in negative control group (55. 6±3. 6 invasive cells per field), blank transfection group (60. 8±7. 4 invasive cells per field) and blank control group (61.6±8. 6 invasive cells per field, P＜0. 01). Conelusion Up-regulation of miR-451 expression may inhibit the bioactivity of colon cancer cells, and it may be attributed to the inhibition of MIF expression.关键词
MIRN451微小RNA,人/结直肠肿瘤/巨噬细胞游走抑制因子Key words
MIRN451 microRNA/ human/ colorectal neoplasms/ macrophage migration-inhibitory factors分类
医药卫生引用本文复制引用
吴春蓉,罗治彬,陈伟..miR-451对结肠癌细胞系SW620生物学行为的影响[J].解放军医学杂志,2011,36(5):478-482,5.基金项目
重庆市卫生局2010年度医学科研计划重点项目(2010-1-71) （2010-1-71）
