中国水产科学2011，Vol.18Issue(3)：565-573,9.DOI:10.3724/SP.J.1118.2011.00565
利用SSR、EST-SSR、SNP标记对鲤食物转化率、体厚、体质量3种性状的分析
Analysis of feed conversion ratio,bodyweight,body thickness traits of common carp (Cyprinus carpio L.) using SSR,EST-SSR,SNP markers
摘要
Abstract
An F2 hybrid common carp gynogenetic line including 68 individuals was used as mapping panel.Markers were used to make marker regression analysis based on pseudo-testcross strategy using Windows Map Manager2.0.Ninety-one SSR markers and 33 EST makers and 364 SNP markers were selected to analyze the genomic DNA of 68 progenies derived from common carp full-sib family.Twenty-seven loci were discovered that had significant impact on feed conversion efficiency and thirty-five loci were significantly related with body thickness (P＜0.05), while twenty seven loci had significant impact on body weight (P＜0.05).SNP0041 and SNP0044 were related with feed conversion ratio significantly (P＜0.01), contributing to 15％ and 16％, respectively.These markers can be used as molecular marker assisting breeding.Markers were used to screen the protein and nucleotide database in the National Center for Biotechnology Information (NCBI) in order to find the homologies.Blasting results showed that HLJ253 is highly correlated (61％) with the gene encoding structural protein (ORF2) of zebrafish.SNP makers associated with three traits were sequenced on NCBI in order to find related genes and possible functions.关键词
鲤/EST-SSR/SNP/食物转化率/体厚/体质量Key words
Cyprinus carpio L./ EST-SSR/ SNP/ feed conversion ratio/ body thickness/ body weight分类
农业科技引用本文复制引用
王宣朋,张晓峰,李文升,张天奇,孙效文..利用SSR、EST-SSR、SNP标记对鲤食物转化率、体厚、体质量3种性状的分析[J].中国水产科学,2011,18(3):565-573,9.基金项目
国家973计划项目(2010CB126305) （2010CB126305）
农业部公益性行业科研专项(200903045). （200903045）
