中国水产科学2011，Vol.18Issue(3)：574-580,7.DOI:10.3724/SP.J.1118.2011.00574
栉孔扇贝、虾夷扇贝及其杂交子代的群体遗传多样性分析
Genetic variation of Chlamys farreri,Patinopecten yessoensis and their hybrids
摘要
Abstract
Amplified fragment length polymorphism (AFLP) technology was used to analyze the genetic variation and genetic structure among three populations of Chlamys farreri(♀), Patinopecten yessoensis(♂)and their offspring F1. In total, 315 loci were amplified with five primer pairs, and 311 of these loci were polymorphic, accounting for 98.73％. Shannon genetic diversity indices were 0.477 1±0.260 6, 0.423 9±0.277 7 and 0.548 8±0.220 9, respectively for the female parent, the male parent and F1, while Nei's gene diversities were 0.329 1±0.185 0, 0.289 7±0.195 9 and 0.382 8±0.161 2, respectively. The genetic distance between C. farreri and P. yessoensis was 0.411 6, while it was 0.131 6 between F1 and their female parents, and 0.278 0 between F1 and their male parents. The dendrograms showed that the three population gathered independently, which indicated that each population was independent. AMOVA analysis indicated that 30.47％ of variance was among populations and 69.53％ variance was within populations, which suggested rich genetic diversity level within populations. In all, the expression of heterosis has a correlation with the genetic variation.关键词
栉孔扇贝/虾夷扇贝/F1/AFLP/遗传多样性/杂种优势Key words
Chlamysfarreri/ patinopecten yessoensis/ F1/ AFLP/ population/ genetic variation/ heterosis分类
农业科技引用本文复制引用
于涛,杨爱国,周丽青,吴彪,刘志鸿..栉孔扇贝、虾夷扇贝及其杂交子代的群体遗传多样性分析[J].中国水产科学,2011,18(3):574-580,7.基金项目
国家863计划项目(2006AA10A408) （2006AA10A408）
国家科技支撑计划专题项目(2006BAD01A00) （2006BAD01A00）
山东省自然科学资金青年基金项目(ZR2009DQ006) （ZR2009DQ006）
青岛市科技计划基础研究项目(09-1-3-12-jch) （09-1-3-12-jch）
中央级公益性基本科研业务费专向资助项目(2009-chb-06). （2009-chb-06）
