中国水产科学2011，Vol.18Issue(3)：581-587,7.DOI:10.3724/SP.J.1118.2011.00581
低渗诱导太平洋牡蛎三倍体以及与其他诱导方法的比较
Triploid induction in Pacific oyster (Crassostrea gigas) by hypotonic treatment and comparison with other induction methods
摘要
Abstract
Triploid Pacific oysters (Crassostrea gigas) were induced by inhibiting polar body Ⅱ (PB2) releasing with hypotonic treatment.Different hypotonic treatments, including salinity (4, 6, 8, 10, 12, 14 and 16), initial treatment time (at the time when the first PB 1, 30 percents of PB 1, 40 percents of PB 1, 50 percents of PB 1 and the first PB2 were observed) and duration time (10, 15, 20 and 25 min) were tested at 23-25℃ water temperature.Flow cytometry analysis showed that the highest triploidy rate was (89.16±1.39)％ when the fertilized eggs were treated for 15 min with hypotonic solution with salinity of 8 at the time when 40 percents of PB 1 appeared.The results also showed that shell height of triploids were larger than that of diploids.And triploid larvae appeared much superiority in growth.Then four different triploid induction methods were compared including hypotonic treatment, cold shock (2 ℃), heat shock (32 ℃) and 6-DMAP (450 μmol/L) treatment.The triploids induced by hypotonic treatment were superior over those by cold shock, heat shock and 6-DMAP, and the difference in hatching success and D-larval size was significant (P＜0.05) The induction ratio of 6-DMAP treatment was higher than that by hypotonic treatment, but the difference was not significant (P＞0.05).General evaluation index (Ie) of hypotonic treatment is superior over those of other three triploid induction treatments.Because of the low price,the high induction ratio of triploids and security, it is feasible and worthwhile to induce triploid by hypotonic treatment.关键词
太平洋牡蛎/三倍体/低渗诱导Key words
Crassostrea gigas/ triploidy/ hypotonic treatment分类
农业科技引用本文复制引用
孔静,王昭萍,于瑞海,刘剑,张跃环..低渗诱导太平洋牡蛎三倍体以及与其他诱导方法的比较[J].中国水产科学,2011,18(3):581-587,7.基金项目
国家自然科学基金项目(30771622) （30771622）
国家高技术研究发展规划项目(2006AA10A401). （2006AA10A401）
