中国水产科学2011，Vol.18Issue(3)：646-653,8.DOI:10.3724/SP.J.1118.2011.00646
中国明对虾抗菌肽基因应答WSSV侵染的表达及其SNP分析
Expression of three kinds of peneaidins after WSSV challenge and their SNPs detection in Chinese shrimp,Fenneropenaeus chinensis
摘要
Abstract
According to gene sequences, penaeidins (one kind of antibacterial peptide in shrimps) in genome of Chinese shrimp, Fenneropenaeus chinensis, could be assorted into three kinds. In order to explore the expression differences among-the three penaeidins after WSSV challenge, real-time quantitative PCR was used to detect the expression characteristics of these penaeidins at different time after WSSV challenge in Chinese shrimp. Shrimps used in the experiment came from the same parentage. After fed each individual the same dose of WSSV, 3-6 samples were collected at 0 h (uninfected), 6 h, 12 h, 24 h, 48 h, 72 h, 96 h, 120 h and 156 h after infection. The individuals dying in three days were regarded as WSSV-susceptive population, while those still living after seven days were regarded as anti-WSSV population. The results showed that the expression of penaeidins was tissue specific in four tissues of hepatopancreases, muscles, gills, and intestines, and the richness of expression and expression tendency from 0 h to 156 h was significantly different (P＜0.05). However, as far as one tissue was concerned, the tendency of expression for three penaeidins was similar in the period from 0 to 120 hours after challenge. For example, the expression of three penaeidins' mRNA in hepatopancreas was 0 at 0 h, and increased at 6-24 h, then decreased at 48-120 h, however increased again at 156 h. The results suggest that the three kinds of penaeidins have the similar biology function, though their DNA sequences were different from each other. Additionally, in order to study the correlation between penaeidins and anti-WSSV character, SNPs of each penaeidin were screened using PCR-SSCP. No significant correlation was found between SNPs and anti-WSSV or between SNPs and WSSV-susceptive shrimps (P＞0.05). It seemed that the penaeidins of shrimps might mainly take part in resisting bacteria, not in resisting WSSV.关键词
中国明对虾/对虾肽/SNP/表达差异/WSSVKey words
Fenneropenaeus chinensis/ penaeidin/ SNP/ expression difference/ WSSV分类
农业科技引用本文复制引用
高焕,赖晓芳,孟宪红,罗坤,孔杰..中国明对虾抗菌肽基因应答WSSV侵染的表达及其SNP分析[J].中国水产科学,2011,18(3):646-653,8.基金项目
国家自然科学基金项目(31072206,310722) （31072206,310722）
中国博士后科学基金(20090461273) （20090461273）
江苏省自然科学基金(SBK200930340). （SBK200930340）
