中国水产科学2011，Vol.18Issue(3)：682-688,7.DOI:10.3724/SP.J.1118.2011.00682
吉富罗非鱼IGF-1基因的基因型对生长和体型的影响
Effects of IGF-1 genotype on weight gain and body shape in GIFT strain Oreochromis niloticus
摘要
Abstract
Insulin-like Growth Factor 1 (IGF-1) is one of important genes controlling growth and development in animals. By comparing with exon1- partial intron 2- exon 3- partial intron 4 sequences of IGF-1 from six individuals of Genetically Improved Farmed Tilapia(GIFT) strain, we identified three SNPs on IGF-1 in this study,including intron 1_A 1307G, intron 1_G1319T and intron3_C6T. PCR-RFLP was performed in intron 1_A1307G and intron3_C6T genotypes detection on 121 individuals from five families of GIFT strain. The results of correlation analysis showed that intron1_Al307G was significantly associated with female fish weight gain and the ratio of body thickness/length (P＜0.05). The weight gain and thickness/length ratio of female fish with AA genotype were significantly higher than those of female fish with GG type (P＜0.05). However, the correlation of genotypes with those traits in male fish was not significant(P＞0.05). Intron3_C6T was significantly associated with weight gain in both male (P＜0.05) and female (P＜0.01). The weight gain of male fish with TT and CT genotype was significantly higher than that of male fish with CC type, while the weight gain of female with CT genotypes was significantly higher than that of female fish with TT. Intron3_C6T was also significantly associated with height/length ratio(P＜0.05) in female fish. The height/length ratio of female fish with TT and CT genotypes was significantly higher than that with CC(P＜0.05). In order to verify the performance of the screened markers in other families, we detected the correlation between intron 1_ A1307G, intron3_C6T genotypes and weight gain among 417 individuals from 60 families of GIFT strain(averaging seven individuals per family), and similar results were observed. The diverse genetic background and smaller size of samples resulted in the decreased correlation significance. These results showed that genetic background and sample size should be considered during molecular maker screening.And the two molecular markers of intronl_ A1307G, intron3_C6T identified in this study could be used as reference in GIFT strain breeding.关键词
胰岛素生长因子-1/吉富罗非鱼/单核苷酸多态性/增重/体型/相关性Key words
IGF-1 gene/ GIFT/ SNP/ weight gain/ body shape/ correlation分类
农业科技引用本文复制引用
阮瑞霞,俞菊华,李红霞,李建林,唐永凯..吉富罗非鱼IGF-1基因的基因型对生长和体型的影响[J].中国水产科学,2011,18(3):682-688,7.基金项目
农业部公益性行业科研专项(200903046-02) （200903046-02）
中央级公益性科研院所基本科研业务费专项资金项目(2011JBFC03) （2011JBFC03）
农业部淡水生物多样性保护与利用重点开放实验室第七期开放课题(LFBU0706). （LFBU0706）
