安徽农业科学2011，Vol.39Issue(14)：8290-8292,3.
冬虫夏草菌种保藏方法的比较
Comparative Study on the Preservation Methods of Cordyceps sinensis Culture
摘要
Abstract
[ Objective ] To compare the preservation effects of three long-term preservation methods on inhibiting the metabolic activity of Cordyceps sinensis culture. [ Method ] With the growth of mycelium,polysaccharide of Cordyceps sinensis and cordycepic acid as indicies, the effects of cryogenic ,liquid nitrogen freezing and freeze-drying on conserving C. sinensis culture of C -03 were comparatively studied. [ Result ] The C.Sinensis culture through vacuum freeze-drying preservation due to skimmed milk and phased cooling of 4 ℃ for 30 min, - 20 ℃ and - 80 ℃freezing for 2 h and 24 h respectively was growing well when germination, the yield of mycelial growth and active ingredients were relatively higher than the other two methods. [ Conclusion] Vacuum freeze-drying preservation was the desirable method to preserve C. sinensis culture.关键词
冬虫夏草菌种/保藏方法/菌丝体生长得率/虫草多糖/虫草酸Key words
Cordyceps sinensis culture/ Preservation method/Yield of mycelial growth/ Polysaccharide ofCordyceps sinensis/Cordycepic acid分类
农业科技引用本文复制引用
方苏,林勤,倪志婧..冬虫夏草菌种保藏方法的比较[J].安徽农业科学,2011,39(14):8290-8292,3.基金项目
国家民委重点实验室项目(2007SY005) （2007SY005）
北方民族大学科研项目(2004Y031). （2004Y031）
