安徽农业科学2011，Vol.39Issue(14)：8191-8193,8240,4.
环介导等温扩增技术快速检测志贺氏菌的研究
Research on the Detection of Shigella by LAMP
摘要
Abstract
[Objective] The aim was to detect Shigella by using the loop-mediated isothermal amplification (LAMP) technology. [Method] The sequences of ipaH of Shigella was used as target sequences to design primers,the reaction conditions such as Mg2+ concentration,Bst DNA polymerase concentration were optimized for establishing LAMP system. [ Result ] The reaction conditions were optimized and the sensitivity of LAMP assay was 62 cfu/ml. [ Conclusion ] LAMP technology for detection of Shigella was developed,and a technology platform was constructed for the rapid detection of shigella in food.关键词
环介导等温扩增技术/检测/志贺氏菌Key words
LAMP/ Detection /Shigella分类
农业科技引用本文复制引用
马晓燕,张会彦,宋明明,张先舟,王羽,张伟..环介导等温扩增技术快速检测志贺氏菌的研究[J].安徽农业科学,2011,39(14):8191-8193,8240,4.基金项目
河北省自然科学基金项目(C2008000216). （C2008000216）
