四川大学学报（医学版）2011，Vol.42Issue(3)：344-348,5.
应用微阵列芯片分析结肠癌组织中miRNA差异性表达
Differential Expression of Colon Cancer MicroRNA in Microarry Study
摘要
Abstract
Objective To investigate the miRNA expression difference between colon cancer and normal colonic mucosa. Methods Twelve (12) pairs of colorectal cancer tissue and normal colonic mucosa were collected,total RNA was extracted and purified. After fluorescent tags being added, hybridization was carried out on miRNA microarray chip (Affymetrix company). SAM analysis was performed to find out the significant expression difference, then the difference was verified by RT-PCR, finally target gene analysis software was utilized to predict the miRNA function. Results The up-regulated miRNAs in colon cancer included has-miR-182, has-miR-17, hasmiR-106a, has-miR-93, has-miR-200c, has-miR-92a, has-let-7a, has-miR-20a (FDR value＜5％), while the downregulated miRNAs were has-miR-195, has-miR-143, has-miR-145 (FDR value ＜ 50％). Conclusion There is significant difference of miRNA expression between colon caner and normal colonic mucosa.关键词
结肠癌/miRNA/微阵列芯片Key words
Colon cancer/ MicroRNA/ Microarray引用本文复制引用
马钦,杨烈,王存,于永扬,周斌,周总光..应用微阵列芯片分析结肠癌组织中miRNA差异性表达[J].四川大学学报（医学版）,2011,42(3):344-348,5.基金项目
国家自然科学基金(批准号30830100、30801332)资助 （批准号30830100、30801332）
