云南农业大学学报2011，Vol.26Issue(3)：312-316,5.DOI:10.3969/j.issn.1004-390X(n).2011.03.005
水牛催乳素基因第3外显子群体遗传特征分析
Genetic Characteristics on the Exon 3 of Prolactin Gene in Buffalo (Bubalus bubalus)
摘要
Abstract
The exon 3 of prolactin gene had significant effect on yield of milk, milk fat and milk protein in cow, but the genetic characteristics and the relevance between milk production traits and gene mutation on the exon 3 of prolactin gene in water buffalo are unclear.In the present study, the sequence features of the exon 3 in prolactin gene were examined in 275 individuals which came from 12 river and swamp type buffalo populations using PCR-SSCP and PCR production direct sequencing methods.The results showed that the sequence of exon 3 in prolactin gene was consists of 108 nucleo-tides, and the sequence coding for 36 amino acid residues, the SNPs loci wasn't detected in water buffalo , so there was irrelevance between milk yield traits and the exon 3 of prolactin gene in water buffalo.The fragment had high conservative character in different species by sequences alignment.Twosubstitution was detected in intron 2 of prolactin gene, one is G ＞ A substitution at the 2 265 th nucleo-tide and another is C ＞T substitution at the 2 335 th nucleotide.Moreover, the C ＞T substitution at the 2 335 th nucleotide was only detected in 2 Binlangjiang water buffaloes.关键词
河流型水牛/沼泽型水牛/催乳素/exon 3/PCR-SSCPKey words
river buffalo/ swamp buffalo/ prolactin/ exon 3/ PCR-SSCP分类
农业科技引用本文复制引用
袁峰,苗永旺,李大林,刘丽仙,熊飞,刘伟,贡潘偏抽..水牛催乳素基因第3外显子群体遗传特征分析[J].云南农业大学学报,2011,26(3):312-316,5.基金项目
云南省应用基础研究重点项目(2007C0003Z) （2007C0003Z）
国家自然科学基金项目(30660024) （30660024）
云南省应用基础研究计划面上项目(2006C0034M,2010ZC243) （2006C0034M,2010ZC243）
国家高技术研究发展计划("863"计划)项目(2008AA101001) （"863"计划）
云南省教育厅科学研究基金项目(2010Y344,09C0197). （2010Y344,09C0197）
