中国医科大学学报2011，Vol.40Issue(6)：481-483,3.DOI:CNKI:21-1227/R.20110602.1639.001
P38信号通路对大鼠神经干细胞增殖的影响
Effects of P38 Signaling Pathway On the Proliferation of Rat Neural Stem Cells
摘要
Abstract
Objective To study the effects of P38 signaling pathway on the proliferation of neural stem cells (NSCs) from rat hippocampus. Methods NSCs were cultured in vitro and were divided into control and experimental groups. The control group was cultured in the NSCs media while the experimental group was cultured in NSCs media added with P38 inhibitor,SB203580. The expression of P38 and phosphorylated P38 was detected by Western blot and the proliferation of NSCs was evaluated by MTT. Results Compared with the control group,the expression of phosphorylated P38 in NSCs was significantly lower. Gradual increase of the NSC spheres was observed in the experimental group, and MTT showed that proliferation speed of NSCs was significantly faster in the experimental group (P ＜ 0.05 ). Conclusion The proliferation of neural stem cells significantly increases when P38 signal transduction pathway is inhibited.关键词
神经干细胞/增殖/P38信号转导通路/新生大鼠Key words
neural stem cells/ proliferation/ P38 signaling pathway/ neonatal rats分类
医药卫生引用本文复制引用
佟雷,王振宇,季丽莉,佟晓杰..P38信号通路对大鼠神经干细胞增殖的影响[J].中国医科大学学报,2011,40(6):481-483,3.基金项目
国家自然科学基金资助项目(81070011) （81070011）
辽宁省科技厅社会发展攻关计划(2010225029) （2010225029）
沈阳市科学技术项目计划(F10-149-9-46) （F10-149-9-46）
