中国医科大学学报2011，Vol.40Issue(6)：501-503,3.DOI:CNKI:21-1227/R.20110602.1649.030
改良脊髓缺血后处理对兔缺血/再灌注损伤脊髓的保护作用
Neuroprotective Effects of Modified Ischemic Postconditioning on Spinal Cord Injury Induced by Ischemia Reperfusion in Rabbits
摘要
Abstract
Objective To establish the animal model of modified ischemia-reperfusion postconditioning and study on the neuroprotective effect of modified ischemic postconditioning on the Ischemia reperfusion (IR) Injury. Methods Japanese white rabbits weighing between 2.0 to 2.5 kg were divided into 4 groups:the same surgical procedure without aortic occlusion (group S), the control group (group C ), the standard postconditioning group (group PA ) and the modified postconditioning group (group PB). Twelve rabbits were assigned randomly to each group. Mean blood pressure (MBP),heart rate (HR) and arterial blood gas including pH,PaO2,PaCO2 and lactic acid (Lac) were measured. Functional evaluation with Tarlov scoring was done for the hind limbs 1,3,7 and 28 days postoperative. Morphologically normal motor neurons were counted,and measurements of MDA and SOD levels were performed. Results There was no significant difference among all the groups in MBP,HR,PaO2,PaCO2,Lac and intra-operative body temperature (P＞ 0.05). Tarlov scores of PA and PB groups were significantly higher than those of C group 1,3,7,and 28 days after spinal cord ischemia-reperfusion. The number of normal neurons in PA and PB groups were markedly higher than that of C group (P ＜ 0.05). MDA markedly increased whereas SOD decreased in C group (P ＜0.05 ).Conclusion The model of modified ischemia-reperfusion postconditioning was successfully established; Standard and modified postconditioning methods exerted comparable neuroprotective effects on the spinal cord injury caused by ischemia-reperfusion.关键词
改良缺血后处理/脊髓/缺血/再灌注损伤Key words
modified postconditioning/ spinal cord/ ischemia/reperfusion injury分类
医药卫生引用本文复制引用
艾春雨,阎雪晶,江晓菁,马虹,王俊科..改良脊髓缺血后处理对兔缺血/再灌注损伤脊髓的保护作用[J].中国医科大学学报,2011,40(6):501-503,3.基金项目
国家自然科学基金资助项目(30740092) （30740092）
