云南农业大学学报2011，Vol.26Issue(3)：345-347,363,4.DOI:10.3969/j.issn.1004-390X(n).2011.03.011
不同浓度6-BA和AgNO3对甘蓝叶片不定芽分化的影响
Effect of 6-BA and AgNO3 on Shoot Regeneration of Cabbage Leaf
摘要
Abstract
Transplastomic transformation has become attractive technology in plant molecular breeding.However, the efficiency of plasmid transformation limited because of low regeneration in many plants.Ag-NO3 as a competitive inhibitor regulating the synthesis of ethylene and polyamine plays a very important role in organogenesis or morphogenesis of in vitro cultures.The research was conducted to study the effects of different concentrations of 6-benzylaminopurine (6-BA) and AgNO3 on inducing adventitious buds from cabbage (Brassica oleracea var.capitata L.) leaf.In the experiment, cabbage leaf of aseptic seedling was cut into segments measured about 0.5 cm ×0.5 cm, cultured on the MS medium supplemented with different concentrations of 6-BA and AgNO3.The results showed that the optimal induction medium was MS +6-BA 1 mg/L + AgNO3 0.01 mg/L, the inducing rate reached as high as 96.7％.While the optimal proliferation medium was MS +6-BA 1 mg/L + AgNO3 0.05 mg/L, proliferation rate was 7.99.A high efficiency regeneration system of leaf explants in cabbage has been developed based on the study, which lay the foundation for further research of cabbage chloroplast transformation.关键词
甘蓝/6-BA/AgNO3/不定芽分化Key words
cabbage/ 6-benzylaminopurine/ AgNO3/ adventitious bud differentiation分类
农业科技引用本文复制引用
何雍琴,迟淑娟,杨正安,王仕玉,邵珠华,张应华..不同浓度6-BA和AgNO3对甘蓝叶片不定芽分化的影响[J].云南农业大学学报,2011,26(3):345-347,363,4.基金项目
国家自然科学基金(30972012) （30972012）
云南省自然科学基金(2007C205M). （2007C205M）
