云南农业大学学报2011,Vol.26Issue(3):359-363,5.DOI:10.3969/j.issn.1004-390X(n).2011.03.014
小叶龙竹的快速繁殖与离体保存
Rapid Propagation and Reservation in vitro of Dendrocalamus barbatus Hsueh et D.Z.Li
摘要
Abstract
The test-tube plantlets cultured from the seeds of Dendrocalamus barbatus Hsueh et D.Z.Li were utilized to investigate the system of rapid propagation and reservation in vitro, the results revealed that the shoot of seedlings was a better one of the explants that could induce shoots propagation, for which the MS medium supplemented with 3 mg/L BA and 0.1 mg/L NAA was the best one and the ratio of propagation can be 1 to 4.4.While the 1/2 MS medium supplemented with 1 mg/L NAA and 1 mg/L IB A was the best root medium in which the rooting ratio can be 93% in 15 days and 100% in 27days.The plantlets could be sustained more than 120 days in the best reservation MS medium in vitro supplemented with 10 mg/L CCC, which also could survive and grow in 50 days in the MS medium supplemented with 3 mg/L BA and 0.1 mg/L NAA.As reported, this research is the latest fruits in the world.关键词
小叶龙竹/快速繁殖/离体保存Key words
Dendrocalamus barbatus Hsueh et D.Z.Li/ rapid propagation/ reservation in vitro分类
农业科技引用本文复制引用
何巨擘,程治英,郭振华,曾千春..小叶龙竹的快速繁殖与离体保存[J].云南农业大学学报,2011,26(3):359-363,5.基金项目
中国西南野生生物种质资源库(0802261411). (0802261411)