肿瘤防治研究2011，Vol.38Issue(5)：495-497,3.DOI:10.3971/j.issn.1000-8578.2011.05.003
IL-15上调NKG2D表达对CIK细胞杀伤活性的增强效应
Enhancement of Cytotoxicity against IL-15 Up-regulate NKG2D Expression on Cytokine-in-duced Killer Cells
摘要
Abstract
Objective To analyse the effects of IL-15 on the expression of NKG2D and the cytotoxicity of cytokine-induced killer (CIK) cells against human esophagus carcinoma cell EC9706 in vitro. Methods Peripheral blood mononuclear cells were isolated from healthy donors then divided into two groups: the control group ( cells were cultured in the presence of IFN-γ, anti-CD3 antibody and IL-2) and IL-15 group (cells were cultured in the presence of IFN-γ, anti-CD3 antibody, IL-2 and 10 ng/ml IL-15). Phenotypic characteristics of CIK cells and the expression of NKG2D were analyzed by flow cytometry. After 14 days culture, cytotoxicity of CIK cells against EC9706 cells was measured by using a standard LDH releasing assay. Effector cells were added to target cells at E: T radios of 20: 1, 30: 1. In blocking experiments, NKG2D monoclonal antibody was added to CIK cells for 15 min before plating at 30: 1 E: T ratio. Results NKG2D molecules were significantly up-regulated during the culture period on CD3+ cells (the CIK cells population) and the CD56+ cells. The differences of NKG2D expressions were significant between IL-45 group and the control group (P＜0. 05). Cytotoxicity of CIK cells treated by the IL-15 group was higher than that of the control group, both at 20: 1 E: T ratios and at 30: 1 E: T ratios (P ＜0. 05). When the NKG2D molecules on CIK cell ular membrance were blocked by the NKG2D monoclonal antibody, the cytolytic activity in the control group cells and IL-15 group cells was significantly inhibited. Conclusion IL-15 up-regulated the expression of NKG2D on CIK cells, which enhanced the NKG2D mediated cytotoxicity against EC9706 cells. CIK cells played a role through the NKG2D molecules.关键词
细胞因子诱导的杀伤细胞/NKG2D/食管癌/细胞治疗/IL-15Key words
Cytokine-induced killer cells/ NKG2D/ Esophagus Carcinoma/ Cell therapy/ IL-15分类
医药卫生引用本文复制引用
梅家转,刘桂举,李瑞君,栗敏,张晓娟..IL-15上调NKG2D表达对CIK细胞杀伤活性的增强效应[J].肿瘤防治研究,2011,38(5):495-497,3.基金项目
郑州市博士创业基金资助项目(郑卫2009175) （郑卫2009175）
