肿瘤防治研究2011，Vol.38Issue(5)：519-523,5.DOI:10.3971/j.issn.1000-8578.2011.05.010
Nucleostemin基因特异性短发夹状干扰RNA在裸鼠移植瘤模型体内的抗白血病作用
Anti-leukemia Effects of Nucleostemin Specific Short Hairpin RNA(NS-shRNA)in Nude Mice Xenograft Tumor Model
摘要
Abstract
Objective To investigate the anti-leukemia role of Nucleostemin(NS)specific short hairpin RNA (NS-shRNA) in nude mice xenograft tumor model. Methods HL-60 leukemia cells at logarithmically growing phases were subcutaneouly inoculated into nude mice to make them oncogenic, then cells which were eluted, were cultured to the fifth generation. The invasiveness of HL-60 cells was detected by Transwell test. The heterogeneic nude mice xenograft tumor model of high-oncogenic HL-60 leukemia cells were established through implanting of high-invasive cells. NS-shRNA was synthesized in vitro, and intraperitoneally injected into the mice. The volumes and weights of the tumor were measured, and tumor samples were stained by HE. NS protein expression was detected by immunocytochemistry, and the apoptotic cells of HL-60 were examined by Tunel technique. Results A heterogeneic nude mice xenograft tumor model with high-oncogenic HL-60 leukemia cells were successfully established with uniform tumor volumes. After treated by NS-shRNA for 13 days, the tumor volumes,weight and NS protein in the tumor cells were statistically lower than those in control group. Large destroy of tumor tissue and "apoptosis character" changes appeared in treated group. A great deal of apoptotic cells were observed in tumor tissue after therapy, detected by Tunel technique. Conclusion The anti-leukemia effects of NSshRNA in nude mice xenograft tumor model were significant. One of the mechanisms was probably that down-regulation of NS expression induce the apoptosis of leukemia cells.关键词
核干细胞因子/短发夹状干扰RNA/裸鼠移植瘤/白血病/HL-60细胞Key words
Nucleostemin/ Specific short hairpin RNA/ Nude mice xenograft tumor/ Leukemia/ HL-60 cell分类
医药卫生引用本文复制引用
岳保红,王园园,蔚利纳,付书贞,阚全程..Nucleostemin基因特异性短发夹状干扰RNA在裸鼠移植瘤模型体内的抗白血病作用[J].肿瘤防治研究,2011,38(5):519-523,5.基金项目
中国博士后科研基金资助项目(20090450934) （20090450934）
河南省卫生厅科技攻关项目(0803039) （0803039）
