中医学报2011，Vol.26Issue(5)：579-581,3.
胆总管结扎诱发大鼠肝外胆汁淤积的动态病理变化研究
Study on Dynamic Pathological Change of Extrahepatic Cholestasis in Rats lnduced by Common Bile Duct Ligation
摘要
Abstract
Observing the pathological change characteristics in the model liver tissue of rat cholestasis induced by BDL. Methods:Common bile duct ligation in three segment was performed to establish the cholestasis model out of the rat liver;five rats from the model group and 3 rats from the sham - operation group were taken randomly when the third day, the first week and the second week separately after the common bile duct ligation and were killed for getting the requisite material;the rest rats were all killed when the third week for getting the requisite material; we observed the pathological change of the liver tissue and the degree of the hepatic fibrosis. Results: Compared with the sham - operation group,in the model group when the third day there are some new epithelia like small bile ducts,a few inflammatory cell infiltrations and a little collagen deposition in the portal area and the lobule perimeter. When the first week there are the expanding portal area and the obvious hyperplastic bile duct epithelia like wreath,the increasing collagen deposition around hyperplastic bile duct and a few hepatocyte necroses. When the second week there are continually increasing hyperplastic flat bile duct epithelia with wide duct and incrassation of the peripheral basement membrane and the collagen fiber. When the third week the hyperplastic bile duct epithelia are like the nodules or evolve into the small bile ducts,and collagen fibers proliferate extensively ;small bile ducts, the fibroblast, collagen fibers and the capillary vessels all form into the membrane -like intervals, which are connected, bundled and segmented with the neighbouring intervals;Compared with the hyperlastic bile ducts, the hepatocytes are relatively decreasing;the morphological structures of the rest hepatocytes are still normal. Conclusion: The pathological change of choleasstis in rat liver induced by BDL are characterized with the hyperplasia of the bile duct epithelia, the decrease of hepatocyte and the collagen hyperplasia and deposition,and these pathological change have a positive correlation with the BDL time within 3 weeks.关键词
胆汁淤积/胆管结扎/大鼠/病理组织学Key words
extrahepatic cholestasis/ BDL/ rat/ histopathology分类
医药卫生引用本文复制引用
朱平生,李燕红,王兵,龙爱华..胆总管结扎诱发大鼠肝外胆汁淤积的动态病理变化研究[J].中医学报,2011,26(5):579-581,3.基金项目
国家自然科学基金资助项目(编号:30500672) （编号:30500672）
河南省高校科技创新人才支持计划资助项目(编号:2009HASTIT031) （编号:2009HASTIT031）
河南省自然科学基金资助项目(编号:0511043400) （编号:0511043400）
河南省教育厅自然科学研究计划资助项目(编号:200510471022) （编号:200510471022）
